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• 211. [Article] North Fork John Day Wild and Scenic River management plan environmental assessment, decision notice and finding of no significant impact

  200 pp. Tables, appendices, glossary, maps. "The North Fork John Day River is located in Northeast Oregon on both the Umatilla and Wallowa-Whitman National Forests, starting in the North Fork John Day ...

  Citation × Citation Citation Abstract Copy Title: North Fork John Day Wild and Scenic River management plan environmental assessment, decision notice and finding of no significant impact Author: North Fork John Day Ranger District (Or.), Baker Ranger District (Or.) Year: 2008, 1993 200 pp. Tables, appendices, glossary, maps. "The North Fork John Day River is located in Northeast Oregon on both the Umatilla and Wallowa-Whitman National Forests, starting in the North Fork John Day Wilderness (Section 13, T 8 S, R 36 E) and flowing north and then west to its confluence with Camas Creek (Section 33, T 6 S, R 31 E)." Decision notice is dated September 13, 1993. Captured June 12, 2008. Title: North Fork John Day Wild and Scenic River management plan environmental assessment, decision notice and finding of no significant impact Author: North Fork John Day Ranger District (Or.), Baker Ranger District (Or.) Year: 2008, 1993 200 pp. Tables, appendices, glossary, maps. "The North Fork John Day River is located in Northeast Oregon on both the Umatilla and Wallowa-Whitman National Forests, starting in the North Fork John Day Wilderness (Section 13, T 8 S, R 36 E) and flowing north and then west to its confluence with Camas Creek (Section 33, T 6 S, R 31 E)." Decision notice is dated September 13, 1993. Captured June 12, 2008. Close

• 212. [Article] Upper Charley Subwatershed ecosystem restoration projects

  80 pp. Tables, figures. Sections 11-14, 22-28, and 33-36 of T.9N., R.42E., Sections 8, 17-19, and 30 of T.9N., R.43E., and Sections 3 and 4 of T.8N., R.42E. Captured June 23, 2008.

  Citation × Citation Citation Abstract Copy Title: Upper Charley Subwatershed ecosystem restoration projects Author: Pomeroy Ranger District (Or. and Wash.) Year: 2008, 2000 80 pp. Tables, figures. Sections 11-14, 22-28, and 33-36 of T.9N., R.42E., Sections 8, 17-19, and 30 of T.9N., R.43E., and Sections 3 and 4 of T.8N., R.42E. Captured June 23, 2008. Title: Upper Charley Subwatershed ecosystem restoration projects Author: Pomeroy Ranger District (Or. and Wash.) Year: 2008, 2000 80 pp. Tables, figures. Sections 11-14, 22-28, and 33-36 of T.9N., R.42E., Sections 8, 17-19, and 30 of T.9N., R.43E., and Sections 3 and 4 of T.8N., R.42E. Captured June 23, 2008. Close

• 213. [Article] Umatilla and Meacham ecosystem analysis and Upland forest vegetation analysis: Umatilla and Meacham watersheds

  296 pp. Tables, figures, references, maps, glossary, appendices. "The Umatilla-Meacham ecosystem analysis area is located on the Walla Walla Ranger District of the Umatilla National Forest. The analysis ...

  Citation × Citation Citation Abstract Copy Title: Umatilla and Meacham ecosystem analysis and Upland forest vegetation analysis: Umatilla and Meacham watersheds Author: Umatilla National Forest (Agency : U.S.), Powell, David C. Year: 2008, 2001 296 pp. Tables, figures, references, maps, glossary, appendices. "The Umatilla-Meacham ecosystem analysis area is located on the Walla Walla Ranger District of the Umatilla National Forest. The analysis area lies on the west flank of the Blue Mountains in the headwaters of the Umatilla River subbasin (HUC #17070103), about 25 miles east of Pendleton, Oregon (Figure 1-1). Together, the two watersheds (Umatilla and Meacham) amount to about 200,700 acres, or about 313.5 square miles in area. About 70 percent of the analysis area is National Forest land (Figure 1-2)." Date of Upland forest vegetation analysis is October 1994. Captured June 18, 2008. Title: Umatilla and Meacham ecosystem analysis and Upland forest vegetation analysis: Umatilla and Meacham watersheds Author: Umatilla National Forest (Agency : U.S.), Powell, David C. Year: 2008, 2001 296 pp. Tables, figures, references, maps, glossary, appendices. "The Umatilla-Meacham ecosystem analysis area is located on the Walla Walla Ranger District of the Umatilla National Forest. The analysis area lies on the west flank of the Blue Mountains in the headwaters of the Umatilla River subbasin (HUC #17070103), about 25 miles east of Pendleton, Oregon (Figure 1-1). Together, the two watersheds (Umatilla and Meacham) amount to about 200,700 acres, or about 313.5 square miles in area. About 70 percent of the analysis area is National Forest land (Figure 1-2)." Date of Upland forest vegetation analysis is October 1994. Captured June 18, 2008. Close

• 214. [Article] Phillips-Gordon ecosystem analysis and Upland forest vegetation analysis

  249 pp. Tables, figures, references, maps, appendices, illus. "The analysis area lies within the Upper Grande Ronde (UGR) Watershed (the main stem and all its tributaries upstream of Rondowa), which is ...

  Citation × Citation Citation Abstract Copy Title: Phillips-Gordon ecosystem analysis and Upland forest vegetation analysis Author: Walla Walla Ranger District (Wash.) Year: 2008, 2001 249 pp. Tables, figures, references, maps, appendices, illus. "The analysis area lies within the Upper Grande Ronde (UGR) Watershed (the main stem and all its tributaries upstream of Rondowa), which is one of three subdivisions of the Upper Grande Ronde Subbasin (Figure 1-1, Map Appendix). The analysis area includes two small HUC-5 watersheds, Phillips/Willow Creek (# 84) and Gordon Creek (#07). Eleven subwatersheds are recognized in this analysis, two in the Gordon Creek drainage and nine in Phillips/Willow Creek drainage (Figure 1-2, Map Appendix; Table 1-1)." Date of vegetation analysis is September 2000. Captured June 18, 2008. Title: Phillips-Gordon ecosystem analysis and Upland forest vegetation analysis Author: Walla Walla Ranger District (Wash.) Year: 2008, 2001 249 pp. Tables, figures, references, maps, appendices, illus. "The analysis area lies within the Upper Grande Ronde (UGR) Watershed (the main stem and all its tributaries upstream of Rondowa), which is one of three subdivisions of the Upper Grande Ronde Subbasin (Figure 1-1, Map Appendix). The analysis area includes two small HUC-5 watersheds, Phillips/Willow Creek (# 84) and Gordon Creek (#07). Eleven subwatersheds are recognized in this analysis, two in the Gordon Creek drainage and nine in Phillips/Willow Creek drainage (Figure 1-2, Map Appendix; Table 1-1)." Date of vegetation analysis is September 2000. Captured June 18, 2008. Close

• 215. [Article] Tucannon ecosystem analysis

  264 pp. Tables, figures, references, maps, appendices, illus. "The Tucannon River subbasin covers about 322,000 acres of Garfield and Columbia counties. Two major streams drain this subbasin, the Tucannon ...

  Citation × Citation Citation Abstract Copy Title: Tucannon ecosystem analysis Author: Pomeroy Ranger District (Or. and Wash.) Year: 2008, 2002 264 pp. Tables, figures, references, maps, appendices, illus. "The Tucannon River subbasin covers about 322,000 acres of Garfield and Columbia counties. Two major streams drain this subbasin, the Tucannon River, which flows into the Snake River, and Pataha Creek, which is the largest tributary to the Tucannon River. Other tributaries to the Tucannon River include Willow Creek, Kellogg Creek, Cummings Creek, Little Tucannon River, Panjab Creek, Sheep Creek, and Bear Creek. The upper portion of the Tucannon subbasin, which is the focus of this report, is contained within the Umatilla National Forest." Date of map appendix is August 2002. Captured June 18, 2008. Title: Tucannon ecosystem analysis Author: Pomeroy Ranger District (Or. and Wash.) Year: 2008, 2002 264 pp. Tables, figures, references, maps, appendices, illus. "The Tucannon River subbasin covers about 322,000 acres of Garfield and Columbia counties. Two major streams drain this subbasin, the Tucannon River, which flows into the Snake River, and Pataha Creek, which is the largest tributary to the Tucannon River. Other tributaries to the Tucannon River include Willow Creek, Kellogg Creek, Cummings Creek, Little Tucannon River, Panjab Creek, Sheep Creek, and Bear Creek. The upper portion of the Tucannon subbasin, which is the focus of this report, is contained within the Umatilla National Forest." Date of map appendix is August 2002. Captured June 18, 2008. Close

• 216. [Article] South River commercial thinning 2000 environmental assessment

  65 pp. Tables, figures, references, appendices, maps. The stands are located in Section 19 of T. 28 S., R. 8 W.; Section 19 of T. 29 S., R. 2 W.; Sections 13 and 24 of T. 29 S., R. 3 W.; Section 9 of T. ...

  Citation × Citation Citation Abstract Copy Title: South River commercial thinning 2000 environmental assessment Author: United States. Bureau of Land Management. Roseburg District Office Year: 2008, 2001 65 pp. Tables, figures, references, appendices, maps. The stands are located in Section 19 of T. 28 S., R. 8 W.; Section 19 of T. 29 S., R. 2 W.; Sections 13 and 24 of T. 29 S., R. 3 W.; Section 9 of T. 30 S., R. 2 W.; and Sections 3 and 4 of T. 30 S., R. 3 W. Captured July 26, 2007. Title: South River commercial thinning 2000 environmental assessment Author: United States. Bureau of Land Management. Roseburg District Office Year: 2008, 2001 65 pp. Tables, figures, references, appendices, maps. The stands are located in Section 19 of T. 28 S., R. 8 W.; Section 19 of T. 29 S., R. 2 W.; Sections 13 and 24 of T. 29 S., R. 3 W.; Section 9 of T. 30 S., R. 2 W.; and Sections 3 and 4 of T. 30 S., R. 3 W. Captured July 26, 2007. Close

• 217. [Article] Andrews Management Unit record of decision and resource management plan

  127 pp. Tables, maps, references, appendices. The Bureau of Land Management (BLM) Burns District Office (DO) manages 3,275,694 acres of public land located primarily in Harney County, Southeastern Oregon ...

  Citation × Citation Citation Abstract Copy Title: Andrews Management Unit record of decision and resource management plan Author: United States. Bureau of Land Management. Burns District Year: 2008, 2005 127 pp. Tables, maps, references, appendices. The Bureau of Land Management (BLM) Burns District Office (DO) manages 3,275,694 acres of public land located primarily in Harney County, Southeastern Oregon (Map 1). The Burns District is divided into two Resource Areas (RA): the Andrews RA and the Three Rivers RA. The two RAs are further divided into land contained within the boundary of the Steens Mountain Cooperative Management and Protection Area (CMPA) and those in the Andrews RA outside the CMPA boundary; the latter is titled the Andrews Management Unit (AMU). The AMU covers a total of 1,221,314 acres of public land, and the CMPA covers a total of 428,156 acres of public land (these numbers do not include private, State, or U.S. Fish and Wildlife Service [USFWS] lands). Captured August 6, 2007. Title: Andrews Management Unit record of decision and resource management plan Author: United States. Bureau of Land Management. Burns District Year: 2008, 2005 127 pp. Tables, maps, references, appendices. The Bureau of Land Management (BLM) Burns District Office (DO) manages 3,275,694 acres of public land located primarily in Harney County, Southeastern Oregon (Map 1). The Burns District is divided into two Resource Areas (RA): the Andrews RA and the Three Rivers RA. The two RAs are further divided into land contained within the boundary of the Steens Mountain Cooperative Management and Protection Area (CMPA) and those in the Andrews RA outside the CMPA boundary; the latter is titled the Andrews Management Unit (AMU). The AMU covers a total of 1,221,314 acres of public land, and the CMPA covers a total of 428,156 acres of public land (these numbers do not include private, State, or U.S. Fish and Wildlife Service [USFWS] lands). Captured August 6, 2007. Close

• 218. [Article] Upper Lake Creek special recreation management area recreation area management plan environmental assessment

  43 pp. Tables, references, glossary, figure, maps. The public lands encompass all of Sections 7, 17, 18, and 19 of Township 15 South, Range 6 West; and Sections 7-29 of Township 15 South, Range 7 West; ...

  Citation × Citation Citation Abstract Copy Title: Upper Lake Creek special recreation management area recreation area management plan environmental assessment Author: United States. Bureau of Land Management. Eugene District Year: 2008, 2005 43 pp. Tables, references, glossary, figure, maps. The public lands encompass all of Sections 7, 17, 18, and 19 of Township 15 South, Range 6 West; and Sections 7-29 of Township 15 South, Range 7 West; Willamette Meridian. Captured July 13, 2007. Title: Upper Lake Creek special recreation management area recreation area management plan environmental assessment Author: United States. Bureau of Land Management. Eugene District Year: 2008, 2005 43 pp. Tables, references, glossary, figure, maps. The public lands encompass all of Sections 7, 17, 18, and 19 of Township 15 South, Range 6 West; and Sections 7-29 of Township 15 South, Range 7 West; Willamette Meridian. Captured July 13, 2007. Close

• 219. [Article] CONTEMPORARY GENETIC TOOLS FOR IN VIVO INVESTIGATIONS OF H3K27 DEMETHYLASES IN ZEBRAFISH CARDIOGENESIS

  Dynamic histone modification has emerged as a robust and versatile regulator of gene expression in eukaryotic cells. One such modification, the trimethylation of lysine 27 on histone H3 (H3K27me3) is facilitated ...

  Citation × Citation Citation Abstract Copy Title: CONTEMPORARY GENETIC TOOLS FOR IN VIVO INVESTIGATIONS OF H3K27 DEMETHYLASES IN ZEBRAFISH CARDIOGENESIS Author: Akerberg, Alexander Year: 2016 Dynamic histone modification has emerged as a robust and versatile regulator of gene expression in eukaryotic cells. One such modification, the trimethylation of lysine 27 on histone H3 (H3K27me3) is facilitated by the Polycomb repressive complex 2 (PRC2) and contributes to the localized repression of transcription. Subsequently, lysine specific demethylase Kdm6b (Jmjd3) can relieve the repressive H3K27me3 mark, allowing for transcriptional activation. In vitro studies have suggested a role for Kdm6b during mesodermal and cardiovascular differentiation in mammalian embryonic stem cells; however, this relationship has yet to be characterized in vivo. I utilized the advantages of the zebrafish model to investigate the in vivo roles of Kdm6b-family demethylases during development using a reverse genetic approach. I carried out two independent loss-of-function studies to analyze the role of Kdm6b-family demethylases during embryonic development in zebrafish. By comparing genetic loss-of-function and morpholino-mediated knockdown approaches, I found that morpholino–mediated knockdown of kdm6bb transcript produces off-target effects and does not portray an accurate representation of in vivo function. I then show that, while not required for early cardiogenesis, histone demethylases kdm6ba and kdm6bb function redundantly to promote late stage proliferation during heart ventricle trabeculation. These data reveal a previously unknown functional relationship and support the hypothesis that Kdm6b-family demethylases function primarily during later stages of development. Additionally, my description of morpholino-induced off-target effects supports the need to use extreme caution when interpreting morphant phenotypes. Due to the embryonic lethality exhibited by kdm6b-deficient embryos and the limited tools available for spatiotemporal transgene control in zebrafish, I was unable to investigate demethylase function within adult animals. I attempted to circumvent these limitations by creating an inducible gene expression system that uses tissue-specific transgenes that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. I showed that these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. I then demonstrated how this technology could be used to define developmental windows of gene function by spatiotemporally controlling expression of constitutively active Notch1 in embryos. This dissertation contains previously published co-authored material. Title: CONTEMPORARY GENETIC TOOLS FOR IN VIVO INVESTIGATIONS OF H3K27 DEMETHYLASES IN ZEBRAFISH CARDIOGENESIS Author: Akerberg, Alexander Year: 2016 Dynamic histone modification has emerged as a robust and versatile regulator of gene expression in eukaryotic cells. One such modification, the trimethylation of lysine 27 on histone H3 (H3K27me3) is facilitated by the Polycomb repressive complex 2 (PRC2) and contributes to the localized repression of transcription. Subsequently, lysine specific demethylase Kdm6b (Jmjd3) can relieve the repressive H3K27me3 mark, allowing for transcriptional activation. In vitro studies have suggested a role for Kdm6b during mesodermal and cardiovascular differentiation in mammalian embryonic stem cells; however, this relationship has yet to be characterized in vivo. I utilized the advantages of the zebrafish model to investigate the in vivo roles of Kdm6b-family demethylases during development using a reverse genetic approach. I carried out two independent loss-of-function studies to analyze the role of Kdm6b-family demethylases during embryonic development in zebrafish. By comparing genetic loss-of-function and morpholino-mediated knockdown approaches, I found that morpholino–mediated knockdown of kdm6bb transcript produces off-target effects and does not portray an accurate representation of in vivo function. I then show that, while not required for early cardiogenesis, histone demethylases kdm6ba and kdm6bb function redundantly to promote late stage proliferation during heart ventricle trabeculation. These data reveal a previously unknown functional relationship and support the hypothesis that Kdm6b-family demethylases function primarily during later stages of development. Additionally, my description of morpholino-induced off-target effects supports the need to use extreme caution when interpreting morphant phenotypes. Due to the embryonic lethality exhibited by kdm6b-deficient embryos and the limited tools available for spatiotemporal transgene control in zebrafish, I was unable to investigate demethylase function within adult animals. I attempted to circumvent these limitations by creating an inducible gene expression system that uses tissue-specific transgenes that express the Gal4 transcription factor fused to the estrogen-binding domain of the human estrogen receptor. I showed that these Gal4-ERT driver lines confer rapid, tissue-specific induction of UAS-controlled transgenes following tamoxifen exposure in both embryos and adult fish. I then demonstrated how this technology could be used to define developmental windows of gene function by spatiotemporally controlling expression of constitutively active Notch1 in embryos. This dissertation contains previously published co-authored material. Close

• 220. [Article] Developing Methods Based on Light Sheet Fluorescence Microscopy for Biophysical Investigations of Larval Zebrafish

  Adapting the tools of optical microscopy to the large-scale dynamic systems encountered in the development of multicellular organisms provides a path toward understanding the physical processes necessary ...

  Citation × Citation Citation Abstract Copy Title: Developing Methods Based on Light Sheet Fluorescence Microscopy for Biophysical Investigations of Larval Zebrafish Author: Taormina, Michael Year: 2014 Adapting the tools of optical microscopy to the large-scale dynamic systems encountered in the development of multicellular organisms provides a path toward understanding the physical processes necessary for complex life to form and function. Obtaining quantitatively meaningful results from such systems has been challenging due to difficulty spanning the spatial and temporal scales representative of the whole, while also observing the many individual members from which complex and collective behavior emerges. A three-dimensional imaging technique known as light sheet fluorescence microscopy provides a number of significant benefits for surmounting these challenges and studying developmental systems. A thin plane of fluorescence excitation light is produced such that it coincides with the focal plane of an imaging system, providing rapid acquisition of optically sectioned images that can be used to construct a three-dimensional rendition of a sample. I discuss the implementation of this technique for use in larva of the model vertebrate Danio rerio (zebrafish). The nature of light sheet imaging makes it especially well suited to the study of large systems while maintaining good spatial resolution and minimizing damage to the specimen from excessive exposure to excitation light. I show the results from a comparative study that demonstrates the ability to image certain developmental processes non-destructively, while in contrast confocal microscopy results in abnormal growth due to phototoxicity. I develop the application of light sheet microscopy to the study of a previously inaccessible system: the bacterial colonization of a host organism. Using the technique, we are able to obtain a survey of the intestinal tract of a larval zebrafish and observe the location of microbes as they grow and establish a stable population in an initially germ free fish. Finally, I describe a new technique to measure the fluid viscosity of this intestinal environment in vivo using magnetically driven particles. By imaging such particles as they are oscillated in a frequency chirped field, it is possible to calculate properties such as the viscosity of the material in which they are embedded. Here I provide the first known measurement of intestinal mucus rheology in vivo. This dissertation includes previously published co-authored material. Title: Developing Methods Based on Light Sheet Fluorescence Microscopy for Biophysical Investigations of Larval Zebrafish Author: Taormina, Michael Year: 2014 Adapting the tools of optical microscopy to the large-scale dynamic systems encountered in the development of multicellular organisms provides a path toward understanding the physical processes necessary for complex life to form and function. Obtaining quantitatively meaningful results from such systems has been challenging due to difficulty spanning the spatial and temporal scales representative of the whole, while also observing the many individual members from which complex and collective behavior emerges. A three-dimensional imaging technique known as light sheet fluorescence microscopy provides a number of significant benefits for surmounting these challenges and studying developmental systems. A thin plane of fluorescence excitation light is produced such that it coincides with the focal plane of an imaging system, providing rapid acquisition of optically sectioned images that can be used to construct a three-dimensional rendition of a sample. I discuss the implementation of this technique for use in larva of the model vertebrate Danio rerio (zebrafish). The nature of light sheet imaging makes it especially well suited to the study of large systems while maintaining good spatial resolution and minimizing damage to the specimen from excessive exposure to excitation light. I show the results from a comparative study that demonstrates the ability to image certain developmental processes non-destructively, while in contrast confocal microscopy results in abnormal growth due to phototoxicity. I develop the application of light sheet microscopy to the study of a previously inaccessible system: the bacterial colonization of a host organism. Using the technique, we are able to obtain a survey of the intestinal tract of a larval zebrafish and observe the location of microbes as they grow and establish a stable population in an initially germ free fish. Finally, I describe a new technique to measure the fluid viscosity of this intestinal environment in vivo using magnetically driven particles. By imaging such particles as they are oscillated in a frequency chirped field, it is possible to calculate properties such as the viscosity of the material in which they are embedded. Here I provide the first known measurement of intestinal mucus rheology in vivo. This dissertation includes previously published co-authored material. Close