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501. [Article] Hawaiian duck (Anas wyvilliana) behavior and response to wetland habitat management at Hanalei National Wildlife Refuge on Kaua'i
The endangered Hawaiian Duck (koloa maoli; Anas wyvilliana), a non-migratory and island-endemic species, experienced a significant population decline during the twentieth century due to factors such as ...Citation Citation
- Title:
- Hawaiian duck (Anas wyvilliana) behavior and response to wetland habitat management at Hanalei National Wildlife Refuge on Kaua'i
- Author:
- Malachowski, Christopher P.
The endangered Hawaiian Duck (koloa maoli; Anas wyvilliana), a non-migratory and island-endemic species, experienced a significant population decline during the twentieth century due to factors such as habitat loss, overharvest, introduced mammalian predators, and hybridization with introduced feral Mallards (A. platyrhynchos). A key objective for Hawaiian Duck recovery is to establish a protected and managed network of wetland habitats; however, development of effective habitat management plans is stymied by the lack of information on patterns of habitat use in relation to fundamental resource requirements. Furthermore, many generalizations regarding dabbling duck behavior and resource requirements that guide seasonal wetland management objectives in North America may not apply to tropical regions and island systems. In this thesis, I compare the behavioral repertoire of the Hawaiian Duck with closely related island-endemic waterfowl and migratory North American Anas, I investigate the behavioral response of Hawaiian Ducks to wetland habitat management and taro cultivation, and I examine the effects of environmental, climatic, temporal, and social factors on the activity budgets of Hawaiian Ducks. I conducted instantaneous focal sampling (n = 984 observation sessions; 328.8 hr) throughout the annual cycle from September 2010 to August 2011 at managed wetlands and taro lo'i within Hanalei National Wildlife Refuge (NWR), Kaua'i. I documented 73 specific Hawaiian Duck behaviors in eight broad behavior categories including foraging, maintenance, resting, locomotion, alert, courtship, and intraspecific and interspecific agonistic interactions. I found that the behavioral repertoire of the Hawaiian Duck was similar to that of the Mallard; however, subtle variations in the form and linkage of certain courtship displays, such as nod-swimming, were observed. Additionally, male Hawaiian Ducks were occasionally associated with brood-rearing females (11% of brood observations), and this behavior appeared to be a male strategy whereby females received little perceived benefit , but males may have potentially garnered additional breeding attempts or maintained pair-bonds for subsequent breeding seasons. After accounting for sex, pair status, month, and time of day, the diurnal behavioral activities of Hawaiian Ducks differed between managed wetlands and taro habitats (F₆,₉₆₀ = 30.3, P < 0.001). Hawaiian Ducks utilized taro predominantly for resting (44%), maintenance (21%), and foraging (15%), while birds used managed wetlands for a variety of activities, including foraging (11%), maintenance (28%), resting (27%), and locomotion (22%). Social activities, particularly courtship, occurred more frequently in managed wetlands than in taro (H₁ = 11.9, P < 0.001). In managed wetlands, birds foraged slightly more with increasing cover of Cyperus spp. (r = 0.18, P < 0.001) and Fimbristylis littoralis (r = 0.17, P < 0.01) and decreasing cover of Urochloa mutica (r = -0.15, P < 0.01) and wetland vegetation height (r = -0.22, P < 0.001). Within taro habitat, the behavioral activities of Hawaiian Ducks differed significantly between birds in lo'i and on dikes (F₆,₄₆₈ = 142.8, P < 0.001); birds utilized lo‘i dikes for resting (60%) and maintenance activities (21%), whereas birds entered lo‘i primarily to forage (45%). The activity budget of Hawaiian Ducks was strongly influenced by time of day (F₁₈,₂₇₁₅.₇₈ = 6.4, P < 0.001), and birds spent more time engaged in active behaviors (i.e., foraging, locomotion, and alert) and less time resting during early morning and evening than during late morning and afternoon. While strong seasonal shifts in most behavioral patterns were not detected, males allocated more time to courting (1.1 vs. 0.3%; H1 = 6.92, P = 0.009) and mate-guarding (0.5 vs. <0.1%; H₁ = 9.83, P = 0.002) in managed wetlands between November and March than the remainder of the year. The effects of sex (F₆,₉₆₀ = 6.06, P < 0.001) and social status (F₆,₆₈₂ = 6.69, P < 0.001) on activity budgets were also significant. Females spent more time foraging (18 vs. 12%) and less time in alert, locomotor, and social behaviors than males. Paired birds allocated more time to aggression towards conspecifics, mate-guarding, and courtship, and within taro lo‘i, paired birds foraged more and rested less than unpaired birds. Overall, Hawaiian Duck allocated diurnal activity budgets differently in managed and cultivated wetland habitat at Hanalei NWR, suggesting that both systems may play an important role in fulfilling fundamental daily and seasonal resource requirements. The increased range of activities and foraging tactics used in managed wetlands may indicate the greater habitat diversity (e.g., vegetation structure, patchiness, plant species richness, range of water depths) provided by seasonal wetlands. In general, Hawaiian Duck allocated less time to diurnal foraging than North American Anas, such Mallard and Mottled Duck (A. fulvigula), suggesting that Hawaiian Duck may have lower daily and seasonal energy demands, have access to higher quality diet, or allot more time to nocturnal foraging activities. Also, unlike many North American migratory waterfowl that demonstrate significant behavioral plasticity in adjusting activity budgets to meet seasonal energy demands associated with breeding, molting, wintering, and migration, Hawaiian Duck did not exhibit a strong seasonal shift in most behaviors which may reflect their non-migratory nature and asynchronous life history cycle.
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502. [Article] Evaluating tools used to estimate and manage browse available to wintering moose on the Copper River Delta, Alaska
Ungulates comprise some of the most well researched and intensely managed wildlife populations on earth. As such, they are recognized as ideal study subjects for developing and modifying management tools ...Citation Citation
- Title:
- Evaluating tools used to estimate and manage browse available to wintering moose on the Copper River Delta, Alaska
- Author:
- Smythe, Sharon E.
Ungulates comprise some of the most well researched and intensely managed wildlife populations on earth. As such, they are recognized as ideal study subjects for developing and modifying management tools or theories (Danell et al. 1994, Shipley 2010). An introduced moose (Alces alces gigas) population on the Copper River Delta (CRD; Delta) of south-central Alaska functions as a valuable resource for the residents of Cordova and an isolated research population on which to test managerial techniques. Since its introduction (1949-1958), the founding population of 23 moose has grown to over 830 in 2013, divided into two sub-populations. However, in 1964, the largest earthquake recorded in U.S. history (9.2 magnitude) uplifted the Delta by 1.0-4.0 m, initiating delta-wide changes in hydrology, vegetation distributions, and successional processes. The proportion of stands dominated by woody species, especially alder (Alnus viridis sinuata) and spruce (Picea sitchensis), increased visibly. Furthermore, previous research (1987-89) observed that 90% of the moose on the western region of the Delta wintered within 9-24% of the total land area, possibly restricting their available winter browse. Because moose diets on the CRD are dominated by willows (Salix spp.), managers were concerned that the combined effects of a restricted winter range and earthquake-initiated vegetation changes would negatively influence the population. Managers have responded to this concern by 1) supporting work to estimate the nutritional carrying capacity (NCC; i.e., the forage available to a population within a specified area and time) of the Delta, and 2) by exploring the feasibility of mechanical treatment as a means of stimulating browse production for the moose. Thus, the objectives of this thesis were to 1) explore the factors influencing NCC for moose on the west CRD while combining updated digital and field-collected data to estimate NCC, and 2) to assess the effects of mechanical treatment on the production of moose browse across stand types and over time. We collected field data and evaluated differences in the past (1988-89) and present (2012-13) biomass-predicting regression equations for two willow species (Barclay's and Hooker's willow, Salix barclayi and hookeriana) used within NCC models to determine 1) whether past and present models of Barclay's willow predicted similarly and 2) whether Hooker’s and Barclay's willow differed in average available biomass, nutritional quality, and utilization by moose. The linear coefficients for the current (2013) Barclay’s willow, Hooker's willow, and combined equations were 2.2x, 1.6x, and 1.9x larger, respectively, than that derived from the 1988 model for Barclay’s willow (which possibly included Hooker's willow data). Thus, willows on the CRD may now be supporting more biomass per stem than predicted by prior models. Hooker's and Barclay's willow did not differ in mean available biomass, nutritional values, or utilization rates. These results suggest a need to evaluate the accuracy of older allometric regressions, though separate identification of the visually-similar Barclay's and Hooker's willow may be unnecessary for future biomass-estimating efforts on the CRD. To further explore the factors influencing the biomass available to moose and their associated NCC estimates, we compared 5 NCC model types across 4 winter ranges and under 3 winter-severity scenarios for the western CRD moose population. We conducted a sensitivity analysis (Sx) of our final model to determine the relative influence of factors affecting NCC estimates. Lastly, we compared current (2012-13) browse available biomass, stand type areas (2011), and NCC results to those obtained by past research (1987-89, MacCracken et al. 1997 and 1959/1986, Stephenson et al. 2006) to determine changes over time, while evaluating the effects of models incorporating satellite-based estimates of stand areas and forage nutritional values on NCC estimates. Because recent aerial survey observations suggest expansion of the moose winter range, our final model estimated NCC between 2,198-3,471 moose depending on winter severity within a winter range encompassing the entire west Delta. These results suggest the current western moose population (approximately 600 in 2013) is below NCC. Model components with the largest and smallest Sx were snow depth and tannin- and lignin-caused reductions in forage nutritional quality, respectively. Changes from 1987-2013 in available biomass of forage species ranged from -66-493%, while changes from 1959-2011 in stand type areas ranged from -60-661%. Overall, NCC estimates only declined by 2% from 1959-2013, however inclusion of forage nutritional quality in models reduced NCC estimates by 60%. Lastly, we assessed the use of hydraulic-axing (i.e., hydro-axing) as tool for increasing the available willow biomass. We evaluated treatment effects on biomass, height, nutritional quality (crude protein, lignin, and tannin levels), utilization, and snow burial of the winter forage species within 3 winter-severity scenarios. Sites were treated in 4 winters (1990-92, 2008, 2010, and 2012) within 5 stand types in 20 locations varying from 0.86-63.40 ha in size. Results indicate few significant differences relative to controls, though treatment significantly increased the ratio of willow to alder. Our results may be limited by sample sizes (n = 1-9 per stand type or treatment year) as visual comparison suggests treatment via hydraulic-axing may be an effective method for increasing willow biomass without influencing nutritional quality. However, willows 20-23 years post-treatment are still significantly shorter than untreated willows (P = 0.03). Thus, treatment may result in decreased forage available to moose in severe winters. Management concerns regarding continued earthquake-initiated changes in vegetation distributions and successional processes prompted our investigation. However, studies on the vegetation dynamics of the CRD suggest the vegetation distribution of the Delta may be relatively stable (Boggs 2000, Thilenius 2008). If so, our current estimates of NCC suggest the west Delta can support a larger moose population than is currently present. Hydro-axing may not be necessary to ensure the future of the moose population, though it could be used to counter increases in alder (Alnus viridis sinuata) which are likely within certain successional sequences. However, together with earthquakes, the processes determining the future forage available on the CRD include complex, interactive forces such as glaciers, the Copper River, oceanic tides, and zoological- and human-caused influences. These forces and their effects on the vegetation create a dynamic ecosystem for the moose population, are difficult to predict, and may be further complicated by climate change. As a result, application of any managerial tool may be temporary and often difficult. However, this guarantees a constant need for further revision and redevelopment of the tools used to manage the moose population, ensuring that the moose of the CRD will remain an important resource for researching and refining ungulate management worldwide.
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This seventh annual report outlines our continued progress on each of six objectives. Improved fumigants: We continue to evaluate previously established field tests, which inlicate that chioropicrin continues ...
Citation Citation
- Title:
- Conserving energy by environmentally acceptable practices in maintaining and procuring transmission poles for long service ; August 1987
- Author:
- Morrell, Jeffrey J., Oregon State University. Dept. of Forest Products, Corden, M. E. (Malcolm E.)
This seventh annual report outlines our continued progress on each of six objectives. Improved fumigants: We continue to evaluate previously established field tests, which inlicate that chioropicrin continues to protect Douglas-fir poles after 17 years and piling after 12 years. Vorlex treated poles are being gradually recolonized by decay fungi, while the Vapam treated poles appear to have little resistance to decay fungi. The latter poles were retreated last summer and will be used to determine the effectiveness of fumigant retreatment. Solid methylisothiocyanate (NIT) continues to protect Douglasfir poles, although the 20 percent NIT treatment has experienced slightly higher levels of colonization after 9 years. Additional tests to evaluate the effectiveness of gelatin encapsulated MIT or chioropicrin indicate that both chemicals continue to remain effective. In addition, there now appears to be little difference between the levels of control exhibited following addition of varying amounts of water to the poles along with gelatin encapsulated NIT. Closed tube bioassays indicate the chioropicrin remains at fungitoxic levels after 17 years, while no volatile fungitoxins appear to be present in Vapam or Vorlex treated wood. The evaluation of untreated Douglas-fir posts treated with NIT, chloropicrin, or Vapam indicate that fumigants can not ccatletely protect untreated wood in ground contact unless there is sane other type of preservative treated barrier present. Although the NIT treatment provided the best protection, all of the posts experienced some surface decay and termite attack after 10 years. These results appear similar to those found with more recent tests of posts treated with various coLthinations of preservative containing wraps and fuinigants. Evaluation of Mylone and tridipain, two solid chemicals that degrade to produce NIT, initcates that NIT production and fungal control are enhanced by the addition of basic pH buffers; however, only the pH 12 buffer resulted in rapid fungal control. These results Jnitcate that the rate of fumigant release can be tailored to control specific decay problems. We have also investigated the decomposition of Vapam in wood. As previously reported, there are over 14 potential deccmposition products from this chemical. This past year we developed methods for assaying these chemicals, evaluated the long-term stability of each, and prepared test blocks for evaluating decc*,osjtion in wood. In addition, we have studied the migration of volatile compounds from Vapam and NIT treated blocks under controlled aeration. After 5,000 hours, detectable levesis of NIT, carbon disulfide, and carbon oxysulfide are still present in air surrounding the Douglas-fir blocks. These tests indicate that low levels of volatile chemicals are continuously eninitted from fumigant treated wood. While this poses little difficulty for utility poles, it may pose some hazard for wood in closed spaces. Evaluations will continue until the emission levels decline below detectable limits. In addition to Vapam deccmposition studies, we have also evaluated the decoxrosition, movement and fungitoxicity of NIT under a variety of environmental conditions. In general, wet wood held less NIT, but the degree of control produced was more rapid. These results suggest that dry wood will act as a reseivoir of NIT, which will be released as moisture enters and swells the wood. This effect may provide an excellent long-term decay control strategy. The information from these studies will be used to develop more 111 ecific recaimrndations for fumigant treatment. Cedar Saood Decay Control: This past year we reestablished the field test of promising nei pentachiorophenol replacements, incorporating 26 chemicals into these tests. These saitles will be evaluated after 1 and 2 years to determine efficacy. In addition to the field test, we evaluated 13 new formulations or cinations of formulations in our laboratory screening tests. A nuner of chemicals including Isothiazolone, Amical 48 and a nurrer of quaternary aimnonium compounds appear promising and have been included in the field test. Bolt Hole Decay Prevention: Test established 5 years ago to determine the effectiveness of sprays, liquids or pastes applied to field drilled bolts holes indicate that airanonium bifluoride, Boracol 40, and 10% penta provided greater protection than Polybor or Patox washers. In addition, no evidence of corrosion was associated with any of the treatments. Fumigant treatments below the bolt holes continue to eliminate decay fungi, although samples removed from further down the pole indicate an incoIr)1ete distribution of MIT. These tests will be reevaluated this coming year. Detecting Early Decay and Estimating Residual Strength: We continue to evaluate the use of fluorescent coupled lectins and infra-red spectroscopy for detecting fungi in wood and early decay under controlled laboratory conditions. We have also continued evaluation of longitudinal compression (L) as a measure of ultite wood strength using a series of 27 Ldgepole pine posts. While the dense knot clusters interferred with the analysis, LCS, in combination with other parameters, was a reasonably good predictor of bending strength. These tests will continue with more uniform material. iv In addition to tests of LCS, we have evaluated the ability of small scal e tests to determine the strength of various wood pole connectors. Out results were in close agreement with those obtained using full scale tests aii illustrate the value of using small r1 e tests to develop strength information. Initiation of Decay in Air-Seasoning Douglas-fir: We continue to evaluate the data developed in the air-seasoning st1wies. This past year we began to develop information on the effects of various colony sizes on wood strength. This data will help us assign strength values to the colony size data we ha developed frau the air-seasoning study. At present, only Peniophora spp. has been tested, but . carbonica, P. placenta, and Haematostereum sanguinolentuin will also be included. Evaluation of the teinperatures required to eliminate fungi from Douglas-f ir poles also continue. We have ccatpleted 9 test charges which indicate that the penta treatmants involving a Boulton-seasoning cycle result in a ixre than adequate heating of the wood, while steam treants associated with amnniacal copper arsenate are nre variable. We feel that longer heating periods are required for poles greater than 12 inches in diamater, but that the current 6 hour steam period will result in heating of the center to 67 C for over 1 hour in smaller poles. Microfungi in Douglas-fir Poles: We continue to evaluate the effects of nticrofungi on properties of fumigant treated Douglas-fir poles. These tests indicate that prior colonization of fumigant treated wood by Scytalidium or Trichoderma species resulted in lower weight losses by P. placenta and . carbonica. Evaluation of a Cellon treated Douglas-fir laminated beam indicated that V severe penta depletion was associated with virtually all of the surface decay present. This beam had only been in service for 12 years in an extremely dry climate arxl it is unclear why the decay was so rapid.
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504. [Article] Quantitative Tools for Monitoring Strategy Evaluation and Assessment of Sea Turtle Populations
Green sea turtles, Chelonia mydas, have endangered and threatened populations globally, but several populations show signs of population recovery. In Hawaii, nesting female green turtles have increased ...Citation Citation
- Title:
- Quantitative Tools for Monitoring Strategy Evaluation and Assessment of Sea Turtle Populations
- Author:
- Piacenza, Susan E. H.
Green sea turtles, Chelonia mydas, have endangered and threatened populations globally, but several populations show signs of population recovery. In Hawaii, nesting female green turtles have increased 5.7% year⁻¹ since 1973, but wide fluctuations in census counts of nesting females make recovery diagnosis difficult. For effective management planning, it is critical to have the best information possible on vital rates, and to determine the best tools and practices for incorporating vital rate information, particularly variability, into population models to assess population size and status. Process and observation errors, compounded by late maturity, obscure the relationship between trends on the nesting beach and the entire population. Using sea turtle nesting beach surveys as a population index for assessment is problematic, yet often pragmatic because this is the only population index that is easily accessible. It is advantageous to use a modelling approach that estimates interannual variability in life history traits, accounts for uncertainty from individual-level variability, and allows for population dynamics to emerge from individual behaviors. To this end, I analyzed a long-term data set of marked green sea turtles to determine the degree of temporal variability in key life history traits. From this analysis, I built an agent-based model (ABM) to form the basis of a new assessment tool -- Monitoring Strategy Evaluation. In Chapter 2, I evaluated annual changes in demographic indicators (DIs) of 3,677 nesting green turtles from a 38-year tagging program in the Hawaiian Islands to determine if key life history traits are changing over time and in response to nester density. I used linear mixed models and multistate open robust design models to estimate several DIs and correlated them with nesting female counts. Mean nester carapace length and breeding probability were highly variable over time, suggesting shifts in age structure that could be due to recruitment. The top-ranked model predicted constant female survival over time. A significant positive relationship between the nesting population and breeding probability was evident, and breeding probability shows promise as an indicator of population recovery. This work contributes to a growing set of studies evaluating sea turtle demography for temporal variability and is the first for Hawaiian green turtles. In Chapter 3, I develop the Green Sea Turtle Agent-Based Model (GSTABM) to evaluate how recovery processes differ across disturbance types. The GSTABM incorporates individually variable age-at-maturity, clutch frequency and clutch size, annually variable breeding probability, environmental stochasticity and density dependence in hatchling production. The GSTABM simulates the process of population impact and recovery and the monitoring process, with observation error, on the nesting beach. The GSTABM captures the emergent patterns of interannual nesting variation, nester recruitment, and realistic population growth rates. Changes in survival rates of the nearshore age-stage classes directly affected adult and nester abundance, population growth rate and nester recruitment more than any of the other input parameters. In simulating 100 years of recovery, experimentally disturbed populations began to increase but did not fully return to pre-disturbance levels in adult and nester abundance, population growth or nester recruitment. In simulations with different levels of monitoring effort, adult abundance was poorly estimated, was influenced by population trajectory and disturbance type, and showed marginal improvements in accuracy with increased detection probability. Estimating recruitment showed improvements with increasing detection levels. In the GSTABM, variability in the nesting beach does not mirror variability in adult abundance. The GSTABM is an important tool to determine relationships with monitoring, population assessment, and the underlying biological processes driving changes in the population, and especially, changes on the nesting beach. In Chapter 4, I develop a new simulation-based tool: Monitoring Strategy Evaluation (MoSE) to determine which data source yields the most useful information for population assessments. The MoSE has three main components: the simulated "true" operating, observation, and estimation models. To explore this first use of MoSE, I apply different treatments of disturbance, sampling, and detection to the virtual "true" population, and then sample the nests or nesters, with observation error, to test if the observation "data" accurately diagnose population status indicators. Based on the observed data, I estimated adult abundance, nester recruitment, and population trend and compare them to the known values. I tested the accuracy of the estimated abundance when annually varying inputs of breeding probability, detection and clutch frequency were used instead of constants. I also explored the improvement of trend accuracy with increased study duration. Disturbance type and severity can have important and persistent effects on the accuracy of population assessments drawn from monitoring rookeries. Accuracy in abundance estimates may be most improved by avoiding clutch frequency bias in sampling and including annually varying inputs in the estimation model. Accuracy of nester recruitment may be most improved by increasing detection level and avoiding age-bias in sampling. The accuracy of estimating population trend is most influenced by the underlying population trajectory, disturbance type and disturbance severity. At least 10 years of monitoring data are necessary to accurately estimate population trend, and longer if juvenile age classes were disturbed and trend estimates occur during the recovery phase. The MoSE is an important tool for sea turtle biologists and conservation managers and allows biologists to make informed decisions regarding the best monitoring strategies to employ for sea turtles. This modeling framework is designed to provide an evaluation of monitoring program effectiveness to assist in planning future programs for sea turtles. Altogether, my research suggests certain life history traits of green sea turtles have important temporal variation that should be accounted for in population models, understanding the relationships between nesting and the total population is essential, and basing population assessments from nesting beach data alone is likely to result in incorrect or biased estimates of status indicators. The quantitative tools employed here can be applied to other sea turtle populations and will improve monitoring, and result in better estimates of current population trends and enhance conservation for all species of sea turtles.
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This fifth annual Cooperative Pole Research Program report outlines our progress in the six project objectives. Improved Fumigants Sampling of previously established field tests revealed that Vorlex and ...
Citation Citation
- Title:
- Conserving energy by safe and environmentally acceptable practices in maintaining and procuring transmission poles for long service ; August 1985
- Author:
- Oregon State University, Oregon State University. Dept. of Forest Products
This fifth annual Cooperative Pole Research Program report outlines our progress in the six project objectives. Improved Fumigants Sampling of previously established field tests revealed that Vorlex and Chloropicrin continued to perform well after 15 years, while Vapam was slightly less effective. Solid methylisothiocyanate (MIT) also performed well in the field after 7 years. In additional tests, gelatin encapsulated MIT migrated through Douglas-fir heartwood with addition of moderate quantities of water to degrade the gelatin. However, in the presence of higher quantities of water or no additional water, MIT migration into the wood was slowed. In a previously established test, gelatin encapsulated MIT continues to inhibit reinfestation of poles 3 years after treatment. Pelletized MIT is a new formulation (65% active ingredient) that appears to have some promise. Preliminary tests indicate that up to 95% of the MIT is release in 24 hours, but a small quantity of MIT remains in the pellets after 63 days aeration and may pose a disposal hazard. The solid MIT formulations will permit aboveground applications, increasing the risk that MIT will come in contact with pole hardware. Preliminary tests indicate that MIT had little effect on corrosion of hot dipped, galvanized bolts attached to wood. This suggests that treatment in the crossarm zone with MIT or fumigants that produce MIT should not affect the integrity of attached hardware. i-i In addition to fumigant evaluations, we recently examined an earlier test of groundline treatments with Osmoplastic® and Hollowheart®. After 10 years, these treatments are performing reasonably well, with only a slight rise in the incidence of decay fungi in the past 4 years. We also reevaluated the effectiveness of kerfing for preventing decay and found that this process reduced the depth and width of checks, resulting in a decreased incidence of decay fungi. Kerfing appears to be a valuable method for preventing internal decay at the groundline. Cedar Sapwood Decay Control This past year, the second set of five chemicals applied to control sapwood decay were evaluated after 2 years of exposure. As in earlier evaluations using the Aspergillus bioassay, none of the chemicals approach pentachlorophenol in oil for ability to inhibit sporulation of Aspergillus niger; however, several samples from zones deep in the wood produced a slight zone of effect. This may indicate the presence of a reservoir for long-term protection against decay. Several of the chemicals including Fluor Chrome Arsenic Phenol and Ammoniacal Copper Arsenate (ACA) appear to bind to the wood and may be difficult to detect by the bioassay method. We expect to assess the effectiveness of these treatments using a soil block test. Investigations of the reliability of the Aspergillus bioassay under a variety of conditions indicated that quantity of spores, use of glass or plastic petri dishes, long-term cold storage, and the use of spray inoculum instead of flooding spores had little influence on the bioassay results with pentachiorophenol, Tributyl-tinoxide, or 3 iodo propynyl butylcarbamate; however, incubation temperature did influence assay results. The Aspergillus bioassay is a simple, effective means for estimating residual preservative levels. Bolt Holes Again this year, wood around the unprotected, control bolt holes in pole sections contained such low levels of decay fungi that evaluation of the treated poles will be delayed another year. In addition to the initial bolt hole treatments, we have begun a test to determine if gelatin encapsulated or pelletized MIT can prevent decay development in field-drilled bolt holes. The pole sections used in these tests had already begun to develop decay prior to treatment and will provide an ideal test material. Detecting Decay and Estimatin& Residual Strength of Poles Fluorescent labeled lectins used in our earlier studies detected decay fungi at low weight losses under laboratory conditions. We are currently evaluating this method for detecting fungi in increment cores removed from poles to reduce the need for culturing. Last year we identified a peak that was unique to infrared (IR) spectra of warm water extracts from decayed wood. This past year we attempted to identify the chemical responsible for this peak and found that carbonyl compounds, probably from oxidative lignin degradation, were responsible for the peak. Since brown rot fungi apparently do iv not completely metabolize lignin breakdown products, they accumulate in the decaying wood and can be readily detected by their IR spectra. Strength properties of beams cut front Douglas-fir pole sections, air-seasoned for 3 years significantly decreased although decay fungi could not be uniformly isolated from the beams. In addition, there were gradual declines in work to maximum load and modulus of elasticity, as well as increased Pilodyn pin penetration. These results suggest that some strength losses occurred during air-seasoning; however, the losses were not large and should not endanger pole users. We compared several test methods including the Pilodyn, radial compression tests, longitudinal compression tests, and the pick test for evaluating residual pole strength of the wood surface of Douglas-fir treated with combinations of funtigants or groundline wraps. The results indicate that only the pick test could accurately detect surface damage and illustrate the difficulty of detecting surface damage. This past year we evaluated several sections cut from ACA treated poles stored for a number of years to determine if they were worth salvaging. Static bending tests of beams cut from the ACA treated zone, the treated/untreated boundary, and the inner heartwood revealed ACA treated sapwood had lower MOR and longitudinal compression strength than the other zones. These results represent only a small sample, but they suggest that some strength loss occurs during ACA V treatments. More importantly, the results suggest that we could have reliably predicted beam MOR by testing small plugs removed from the poles. Small beams cut from decaying, pentachlorophenol treated Douglas-fir poles were acoustically tested for residual wood strength, then evaluated to failure in static bending. The acoustic test consisted of sending a pulsed sonic wave into the wood and recording this wave after it passed through the beam. As it moved, the wave was altered by the presence of any wood defects or decay, and these alterations create a "fingerprint" specific for that defect. Preliminary results indicated that signal analysis was highly 2 2 correlated with work to maximum load (r =.82) and MOR (r .88), suggesting that this approach to decay detection may prove more reliable than measuring of sound velocity. Initiation of Decay in Air-Seasoning Douglas-fir The results of the initial survey to determine the incidence of decay fungi in poles from widely scattered Pacific Northwest seasoning yards indicated that a variety of fungi were colonizing the wood. While most of these fungi do not pose a serious decay problem, two species, Poria carbonica and Poria placenta, became increasingly abundant with length of air-seasoning. These fungi are also the most conunon decayers of Douglas-fir poles in service. As expected, the number of fungi and the wood volume they occupied increased with seasoning time; however, this incidence varied considerably between yards, especially in poles air-seasoned for vi shorter time periods. In addition to the variation between sites, many of the decay fungi colonizing the wood appear to be monokaryons, indicating that spores landing on the wood are initiating the infestation. The distribution of fungi within the poles indicated that several of the more abundant decay fungi were present in the outer sapwood where they would be eliminated by conventional pressure treatment. The remaining fungi were most abundant in the heartwood but were more concentrated near the pole end. This suggests that exposed end grain was more readily invaded than lateral grain exposed in checks. In addition to identifying the fungi colonizing Douglas-fir, we examined the effects these fungi had on wood strength. Toughness tests indicated the presence of wide variation in decay capability of the isolates. Although there was no consistent pattern, most of the isolates did not cause substantial decay and, of those that did, only . carbonica and P. placenta were sufficiently abundant to have a large influence on wood strength. Due to the prevalence of P. carbonica and P. placenta in the inner heartwood, where they might not be eliminated in a short heating cycle, we evaluated the temperature tolerance of these two fungi in Douglas-fir heartwood blocks. These tests indicated that both fungi were eliminated by exposure to temperatures above 71°C for over 1 hour or 60°C for 2 hours. The results suggest that careful control of temperature during treatment should eliminate decay fungi and that wood treated at ambient temperatures should be heated to kill fungi that become established during air-seasoning. vii This past year was the third and final year of the decay development study. In this study, sterile pole sections have been exposed for 1, 2, or 3 years at widely scattered Pacific Northwest sites, then returned to the laboratory and extensively sampled. We are now in the process of identifying the fungi from the third year poles. In addition to examining poles prior to preservative treatment, we are also evaluating poles treated with waterborne chemicals (ACA or CCA) for the incidence of surface decay. This past year we examined twenty ACA-treated poles from a line installed in 1946. While a variety of fungi were cultured from the wood, none of the poles had evidence of substantial surface deterioration. A study was initiated on the fungal flora of fumigant treated wood because of the potential for fungi developing resistance to low levels of fumigant or the ability to actively degrade the chemical. Both of these developments could shorten fumigant retreatment cycles and increase maintenance costs. We have evaluated poles treated 7 and 15 years ago with fumigants and find markedly reduced fungal flora. Tests are continuing on the fungi isolated, and we hope to assess the effects of these isolates on long-term fumigant effectiveness.