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• 1. [Article] Engineering a Healthier Watershed: Middle School Students Use Engineering Design to Lessen the Impact of Their Campus' Impervious Surfaces on Their Local Watershed

  It is important that students understand not only how their local watershed functions, but also how it is being impacted by impervious surfaces. Additionally, students need experience exploring the scientific ...

  Citation × Citation Citation Abstract Copy Title: Engineering a Healthier Watershed: Middle School Students Use Engineering Design to Lessen the Impact of Their Campus' Impervious Surfaces on Their Local Watershed Author: Gardner, Elizabeth Claire Year: 2015 It is important that students understand not only how their local watershed functions, but also how it is being impacted by impervious surfaces. Additionally, students need experience exploring the scientific and engineering practices that are necessary for a strong STEM background. With this knowledge students can be empowered to tackle this real and local problem using engineering design, a powerful practice gaining momentum and clarity through its prominence in the recent Framework for K-12 Science Education. Twenty classes of suburban sixth-graders participated in a new five-week Watershed Engineering Design Unit taught by their regular science teachers. Students engaged in scientific inquiry to learn about the structure, function, and health of their local watersheds, focusing on the effects of impervious surfaces. In small groups, students used the engineering design process to propose solutions to lessen the impact of runoff from their school campuses. The goal of this evaluation was to determine the effectiveness of the curriculum in terms of student gains in understanding of (1) watershed function, (2) the impact of impervious surfaces, and (3) the engineering design process. To determine the impact of this curriculum on their learning, students took multiple-choice pre- and post-assessments made up of items covering the three categories above. This data was analyzed for statistical significance using a lower-tailed paired sample t-test. All three objectives showed statistically significant learning gains and the results were used to recommend improvements to the curriculum and the assessment instrument for future iterations. Title: Engineering a Healthier Watershed: Middle School Students Use Engineering Design to Lessen the Impact of Their Campus' Impervious Surfaces on Their Local Watershed Author: Gardner, Elizabeth Claire Year: 2015 It is important that students understand not only how their local watershed functions, but also how it is being impacted by impervious surfaces. Additionally, students need experience exploring the scientific and engineering practices that are necessary for a strong STEM background. With this knowledge students can be empowered to tackle this real and local problem using engineering design, a powerful practice gaining momentum and clarity through its prominence in the recent Framework for K-12 Science Education. Twenty classes of suburban sixth-graders participated in a new five-week Watershed Engineering Design Unit taught by their regular science teachers. Students engaged in scientific inquiry to learn about the structure, function, and health of their local watersheds, focusing on the effects of impervious surfaces. In small groups, students used the engineering design process to propose solutions to lessen the impact of runoff from their school campuses. The goal of this evaluation was to determine the effectiveness of the curriculum in terms of student gains in understanding of (1) watershed function, (2) the impact of impervious surfaces, and (3) the engineering design process. To determine the impact of this curriculum on their learning, students took multiple-choice pre- and post-assessments made up of items covering the three categories above. This data was analyzed for statistical significance using a lower-tailed paired sample t-test. All three objectives showed statistically significant learning gains and the results were used to recommend improvements to the curriculum and the assessment instrument for future iterations. Close

• 2. [Article] Assessing Hydrologic and Water Quality Sensitivities to Precipitation Changes, Urban Growth and Land Management Using SWAT

  Precipitation changes and urban growth are two factors altering the state of water quality. Changes in precipitation will alter the amount and timing of flows, and the corresponding sediment and nutrient ...

  Citation × Citation Citation Abstract Copy Title: Assessing Hydrologic and Water Quality Sensitivities to Precipitation Changes, Urban Growth and Land Management Using SWAT Author: Psaris, Alexander Michael Year: 2014 Precipitation changes and urban growth are two factors altering the state of water quality. Changes in precipitation will alter the amount and timing of flows, and the corresponding sediment and nutrient dynamics. Meanwhile, densification associated with urban growth will create more impervious surfaces which will alter sediment and nutrient loadings. Land and water managers often rely on models to develop possible future scenarios and devise management responses to these projected changes. We use the Soil and Water Assessment Tool (SWAT) to assess the sensitivities of stream flow, sediment, and nutrient loads in two urbanizing watersheds in Northwest Oregon, USA to various climate and urbanization scenarios. We evaluate the spatial patterns climate change and urban growth will have on water, sediment and nutrient yields. We also identify critical source areas (CSAs) and investigate how implementation of vegetative filter strips (VFS) could ameliorate the effects of these changes. Our findings suggest that: 1) Water yield is tightly coupled to precipitation. 2) Large increases in winter and spring precipitation provide enough sub-surface storage to increase summertime water yields despite a moderate decrease in summer precipitation. 3) Expansion of urban areas increases surface runoff and has mixed effects on sediment and nutrients. 4) Implementation of VFS reduces pollutant loads helping overall watershed health. This research demonstrates the usefulness of SWAT in facilitating informed land and water management decisions. Title: Assessing Hydrologic and Water Quality Sensitivities to Precipitation Changes, Urban Growth and Land Management Using SWAT Author: Psaris, Alexander Michael Year: 2014 Precipitation changes and urban growth are two factors altering the state of water quality. Changes in precipitation will alter the amount and timing of flows, and the corresponding sediment and nutrient dynamics. Meanwhile, densification associated with urban growth will create more impervious surfaces which will alter sediment and nutrient loadings. Land and water managers often rely on models to develop possible future scenarios and devise management responses to these projected changes. We use the Soil and Water Assessment Tool (SWAT) to assess the sensitivities of stream flow, sediment, and nutrient loads in two urbanizing watersheds in Northwest Oregon, USA to various climate and urbanization scenarios. We evaluate the spatial patterns climate change and urban growth will have on water, sediment and nutrient yields. We also identify critical source areas (CSAs) and investigate how implementation of vegetative filter strips (VFS) could ameliorate the effects of these changes. Our findings suggest that: 1) Water yield is tightly coupled to precipitation. 2) Large increases in winter and spring precipitation provide enough sub-surface storage to increase summertime water yields despite a moderate decrease in summer precipitation. 3) Expansion of urban areas increases surface runoff and has mixed effects on sediment and nutrients. 4) Implementation of VFS reduces pollutant loads helping overall watershed health. This research demonstrates the usefulness of SWAT in facilitating informed land and water management decisions. Close

• 3. [Article] Continuous Deflection Separation of Stormwater Particulates

  Capture of stormwater particulate matter is of concern to watershed managers trying to meet water quality guidelines by removing toxics associated with particulates from natural water bodies. A laboratory ...

  Citation × Citation Citation Abstract Copy Title: Continuous Deflection Separation of Stormwater Particulates Author: Schwarz, Tracy S., Wells, Scott A. Year: 1999 Capture of stormwater particulate matter is of concern to watershed managers trying to meet water quality guidelines by removing toxics associated with particulates from natural water bodies. A laboratory investigation into particle removal efficiency of a prototype Continuous Deflective Separation (CDS) device was made in the laboratory. Particle size distributions were obtained for roadway drainage surfaces. These distributions were used to assess the predicted performance of a CDS unit in the field. Flow rates in the laboratory tests ranged from 125 GPM (7.9 l/s) to 270 GPM (17.0 l/s). Screen sizes used for testing were 1200 m and 2400 m. Using four different sand types and the two screen sizes, particle capture rates were determined for the CDS unit. Internal velocities were measured at seventy-two measuring positions within the main chamber of the CDS unit. At 270 GPM capture rates based on particle size ranged from 12% to 100% with the 2400 m screen, and 22% to 100% with the 1200 m screen. Cumulative mass capture rates were 73% and 70% for the 1200 m and 2400 m screens, respectively, at 270 gpm and 84% at 125 gpm for the given particle distribution used. Title: Continuous Deflection Separation of Stormwater Particulates Author: Schwarz, Tracy S., Wells, Scott A. Year: 1999 Capture of stormwater particulate matter is of concern to watershed managers trying to meet water quality guidelines by removing toxics associated with particulates from natural water bodies. A laboratory investigation into particle removal efficiency of a prototype Continuous Deflective Separation (CDS) device was made in the laboratory. Particle size distributions were obtained for roadway drainage surfaces. These distributions were used to assess the predicted performance of a CDS unit in the field. Flow rates in the laboratory tests ranged from 125 GPM (7.9 l/s) to 270 GPM (17.0 l/s). Screen sizes used for testing were 1200 m and 2400 m. Using four different sand types and the two screen sizes, particle capture rates were determined for the CDS unit. Internal velocities were measured at seventy-two measuring positions within the main chamber of the CDS unit. At 270 GPM capture rates based on particle size ranged from 12% to 100% with the 2400 m screen, and 22% to 100% with the 1200 m screen. Cumulative mass capture rates were 73% and 70% for the 1200 m and 2400 m screens, respectively, at 270 gpm and 84% at 125 gpm for the given particle distribution used. Close

• 4. [Article] CE-QUAL-W2, Version 3

  CE-QUAL-W2 is a two-dimensional water quality and hydrodynamic code supported by the U.S. Army Engineer Research and Development Center (Cole and Buchak 1995). The model has been widely applied to stratified ...

  Citation × Citation Citation Abstract Copy Title: CE-QUAL-W2, Version 3 Author: Wells, Scott A., Cole, Thomas M. Year: 2000 CE-QUAL-W2 is a two-dimensional water quality and hydrodynamic code supported by the U.S. Army Engineer Research and Development Center (Cole and Buchak 1995). The model has been widely applied to stratified surface water systems such as lakes, reservoirs, and estuaries and computes water levels, horizontal and vertical velocities, temperature, and 21 other water quality parameters (such as dissolved oxygen, nutrients, organic matter, algae, pH, the carbonate cycle, bacteria, and dissolved and suspended solids). A typical model grid is shown in Figure 1 where the vertical axis is aligned with gravity. This technical note documents the development of CE-QUAL-W2, Version 3, which incorporates sloping riverine sections. Version 3 has the capability of modeling entire river basins with rivers and interconnected lakes, reservoirs, and/or estuaries. Four example applications illustrate the use of Version 3. Title: CE-QUAL-W2, Version 3 Author: Wells, Scott A., Cole, Thomas M. Year: 2000 CE-QUAL-W2 is a two-dimensional water quality and hydrodynamic code supported by the U.S. Army Engineer Research and Development Center (Cole and Buchak 1995). The model has been widely applied to stratified surface water systems such as lakes, reservoirs, and estuaries and computes water levels, horizontal and vertical velocities, temperature, and 21 other water quality parameters (such as dissolved oxygen, nutrients, organic matter, algae, pH, the carbonate cycle, bacteria, and dissolved and suspended solids). A typical model grid is shown in Figure 1 where the vertical axis is aligned with gravity. This technical note documents the development of CE-QUAL-W2, Version 3, which incorporates sloping riverine sections. Version 3 has the capability of modeling entire river basins with rivers and interconnected lakes, reservoirs, and/or estuaries. Four example applications illustrate the use of Version 3. Close

• 5. [Article] Validation of the CE-QUAL-W2 Version 3 River Basin Hydrodynamic and Water Quality Model

  CE-QUAL-W2 Version 3, a 2-D (longitudinal-vertical) hydrodynamic and water quality model for river basins combining both river and stratified river-estuary and lakereservoir flow, is a development product ...

  Citation × Citation Citation Abstract Copy Title: Validation of the CE-QUAL-W2 Version 3 River Basin Hydrodynamic and Water Quality Model Author: Wells, Scott A. Year: 2002 CE-QUAL-W2 Version 3, a 2-D (longitudinal-vertical) hydrodynamic and water quality model for river basins combining both river and stratified river-estuary and lakereservoir flow, is a development product of the Waterways Experiment Station in Vicksburg, MS, USA. With the development and release of any revised or reformulated model codes, significant model validation is required. This includes comparison of model results to simple analytical solutions for hydrodynamics and water quality transport, as well as comparison to laboratory and field data. In this paper, the model is compared to numerous analytical solutions for mass transport (1-D advective mass transport) and hydrodynamics (impulsive wind stress on water surface, seiching). Suggestions are presented for proper validation protocols for hydrodynamic and water quality models. Title: Validation of the CE-QUAL-W2 Version 3 River Basin Hydrodynamic and Water Quality Model Author: Wells, Scott A. Year: 2002 CE-QUAL-W2 Version 3, a 2-D (longitudinal-vertical) hydrodynamic and water quality model for river basins combining both river and stratified river-estuary and lakereservoir flow, is a development product of the Waterways Experiment Station in Vicksburg, MS, USA. With the development and release of any revised or reformulated model codes, significant model validation is required. This includes comparison of model results to simple analytical solutions for hydrodynamics and water quality transport, as well as comparison to laboratory and field data. In this paper, the model is compared to numerous analytical solutions for mass transport (1-D advective mass transport) and hydrodynamics (impulsive wind stress on water surface, seiching). Suggestions are presented for proper validation protocols for hydrodynamic and water quality models. Close

• 6. [Article] The Atmosphere can be a Source of Certain Water Soluble Volatile Organic Compounds in Urban Streams

  Surface water and air volatile organic compound (VOC) data from 10 U. S. Geological Survey monitoring sites were used to evaluate the potential for direct transport of VOCs from the atmosphere to urban ...

  Citation × Citation Citation Abstract Copy Title: The Atmosphere can be a Source of Certain Water Soluble Volatile Organic Compounds in Urban Streams Author: Kenner, Scott J, Bender, David A., Pankow, James F., Zogorski, John S. Year: 2014 Surface water and air volatile organic compound (VOC) data from 10 U. S. Geological Survey monitoring sites were used to evaluate the potential for direct transport of VOCs from the atmosphere to urban streams. Analytical results of 87 VOC compounds were screened by evaluating the occurrence and detection levels in both water and air, and equilibrium concentrations in water (C-w(s)) based on the measured air concentrations. Four compounds (acetone, methyl tertiary butyl ether, toluene, and m- & p-xylene) were detected in more than 20% of water samples, in more than 10% of air samples, and more than 10% of detections in air were greater than long-term method detection levels (LTMDL) in water. Benzene was detected in more than 20% of water samples and in more than 10% of air samples. Two percent of benzene detections in air were greater than one-half the LTMDL in water. Six compounds (chloroform, p-isopropyltoluene, methylene chloride, perchloroethene, 1,1,1-trichloroethane, and trichloroethene) were detected in more than 20% of water samples and in more than 10% of air samples. Five VOCs, toluene, m- & p-xylene, methyl tert-butyl ether (MTBE), acetone, and benzene were identified as having sufficiently high concentrations in the atmosphere to be a source to urban streams. MTBE, acetone, and benzene exhibited behavior that was consistent with equilibrium concentrations in the atmosphere. Title: The Atmosphere can be a Source of Certain Water Soluble Volatile Organic Compounds in Urban Streams Author: Kenner, Scott J, Bender, David A., Pankow, James F., Zogorski, John S. Year: 2014 Surface water and air volatile organic compound (VOC) data from 10 U. S. Geological Survey monitoring sites were used to evaluate the potential for direct transport of VOCs from the atmosphere to urban streams. Analytical results of 87 VOC compounds were screened by evaluating the occurrence and detection levels in both water and air, and equilibrium concentrations in water (C-w(s)) based on the measured air concentrations. Four compounds (acetone, methyl tertiary butyl ether, toluene, and m- & p-xylene) were detected in more than 20% of water samples, in more than 10% of air samples, and more than 10% of detections in air were greater than long-term method detection levels (LTMDL) in water. Benzene was detected in more than 20% of water samples and in more than 10% of air samples. Two percent of benzene detections in air were greater than one-half the LTMDL in water. Six compounds (chloroform, p-isopropyltoluene, methylene chloride, perchloroethene, 1,1,1-trichloroethane, and trichloroethene) were detected in more than 20% of water samples and in more than 10% of air samples. Five VOCs, toluene, m- & p-xylene, methyl tert-butyl ether (MTBE), acetone, and benzene were identified as having sufficiently high concentrations in the atmosphere to be a source to urban streams. MTBE, acetone, and benzene exhibited behavior that was consistent with equilibrium concentrations in the atmosphere. Close

• 7. [Article] The Impact of Fine Sediment on Stream Macroinvertebrates in Urban and Rural Oregon Streams

  Urbanization, often characterized by high impervious surface area, can result in excessive inputs of fine sediments into urban streams. Excessive fine sediments can blanket the stream bed filling the interstitial ...

  Citation × Citation Citation Abstract Copy Title: The Impact of Fine Sediment on Stream Macroinvertebrates in Urban and Rural Oregon Streams Author: Hoy, Raymond S. Year: 2001 Urbanization, often characterized by high impervious surface area, can result in excessive inputs of fine sediments into urban streams. Excessive fine sediments can blanket the stream bed filling the interstitial space in the substratum, which may have adverse effects on stream biota. A field survey was conducted in Oregon urban and non-urban basins to investigate the relationship between fine sediments and stream macroinvertebrates. Physical, chemical, and biological data were collected from 59 stream sites in two urban and two rural streams. The stream sites fulfilled a continuous sediment gradient, which ranged from a low of 2% of fine sediment in the substrate to a high of 64% with an average of 22%. The % fines, in Clear Creek (rural basin) was significantly lower than in the urban basins (Johnson Creek and Tryon Creek) (p=0.005). Johnson Creek (mean=23%) had approximately three times more fine sediment than Clear Creek (mean=7%), while Tryon Creek (mean=32%) had nearly five times as much fine sediment as Clear Creek. EPT taxa richness was significantly higher in both rural streams than in both urban streams (p0.05). For example, regression analysis of EPT taxa richness vs. % fine sediments displayed a coefficient of determination (r2) value of 0.2. Other macro invertebrates metrics displayed similar patterns. The lack of significant correlations may be due to the cumulative effect of basin-wide "historical land use past". Past land use activity may have resulted in long-term reductions of sensitive taxa in the basin taxa pool and efforts to improve local habitats may not be quickly colonized by pollution sensitive taxa. Long-term degradation to the urban streams resulted in a relatively homogenous assemblage of macro invertebrates, which may have confounded the quantitative relationship between sediments and macroinvertebrates. This study suggests there is a clear difference between urban and non-urban streams in terms of macro invertebrates, which may be likely due to sediments, but the quantitative relationship between fine sediments and macro invertebrates is weak. Title: The Impact of Fine Sediment on Stream Macroinvertebrates in Urban and Rural Oregon Streams Author: Hoy, Raymond S. Year: 2001 Urbanization, often characterized by high impervious surface area, can result in excessive inputs of fine sediments into urban streams. Excessive fine sediments can blanket the stream bed filling the interstitial space in the substratum, which may have adverse effects on stream biota. A field survey was conducted in Oregon urban and non-urban basins to investigate the relationship between fine sediments and stream macroinvertebrates. Physical, chemical, and biological data were collected from 59 stream sites in two urban and two rural streams. The stream sites fulfilled a continuous sediment gradient, which ranged from a low of 2% of fine sediment in the substrate to a high of 64% with an average of 22%. The % fines, in Clear Creek (rural basin) was significantly lower than in the urban basins (Johnson Creek and Tryon Creek) (p=0.005). Johnson Creek (mean=23%) had approximately three times more fine sediment than Clear Creek (mean=7%), while Tryon Creek (mean=32%) had nearly five times as much fine sediment as Clear Creek. EPT taxa richness was significantly higher in both rural streams than in both urban streams (p0.05). For example, regression analysis of EPT taxa richness vs. % fine sediments displayed a coefficient of determination (r2) value of 0.2. Other macro invertebrates metrics displayed similar patterns. The lack of significant correlations may be due to the cumulative effect of basin-wide "historical land use past". Past land use activity may have resulted in long-term reductions of sensitive taxa in the basin taxa pool and efforts to improve local habitats may not be quickly colonized by pollution sensitive taxa. Long-term degradation to the urban streams resulted in a relatively homogenous assemblage of macro invertebrates, which may have confounded the quantitative relationship between sediments and macroinvertebrates. This study suggests there is a clear difference between urban and non-urban streams in terms of macro invertebrates, which may be likely due to sediments, but the quantitative relationship between fine sediments and macro invertebrates is weak. Close

• 8. [Article] Pharmaceutical Contaminants as Stressors on Rocky Intertidal and Estuarine Organisms: a Case Study of Fluoxetine

  Contaminants such as pharmaceuticals are of increasing concern due to their ubiquitous use and persistence in surface waters worldwide. Limited attention has been paid to the effects of pharmaceuticals ...

  Citation × Citation Citation Abstract Copy Title: Pharmaceutical Contaminants as Stressors on Rocky Intertidal and Estuarine Organisms: a Case Study of Fluoxetine Author: Peters, Joseph Richard Year: 2016 Contaminants such as pharmaceuticals are of increasing concern due to their ubiquitous use and persistence in surface waters worldwide. Limited attention has been paid to the effects of pharmaceuticals on marine life, despite widespread detection of these contaminants in the marine environment. Of the existing studies, the majority assess the negative effects of pharmaceuticals over an exposure period of 30 days or less and focus on cellular and subcellular biomarkers. Longer studies are required to determine if chronic contaminant exposure poses risks to marine life at environmentally relevant concentrations. Also scarce in the literature is examination of whole organism effects to identify potential community-level consequences. Two long-term studies with the antidepressant pharmaceutical, fluoxetine (the active constituent in Prozac®) were conducted to determine whether nominal concentrations detected in estuarine and coastal environments affect organism health and interactions. First, we measured whole organism metrics in the California mussel, Mytilus californianus over a period of 107 days. Specifically, we measured algal clearance rates, growth, and condition indices for both reproductive and overall health. We found that fluoxetine negatively affects all measured characteristics, however many effects are mediated by length of exposure. Perhaps the most notable result was that mussels spiked with fluoxetine cleared less algae after 30 days of exposure. Reduced growth and condition indices likely are a consequence of improper nutrition among fluoxetine-treated mussels. Any level of fluoxetine significantly affected the gonadosomatic index after 47 days. The results from this study on mussels fill an important data gap, highlighting organism-level effects of chronic exposure periods; such data more explicitly identify the impacts of pharmaceuticals and other contaminants on marine communities and ecosystems. Fluoxetine has also been documented to affect the behavior of fish and invertebrates, including freshwater and marine bivalves, crustaceans, and fish. Given that other crustaceans exhibited increased activity levels under fluoxetine exposure, we hypothesized that this would subject them to greater predation risk. In our second exposure study, we assessed whether a similar range of fluoxetine concentrations used in the mussel study altered the risk behavior of the Oregon mud crab, Hemigrapsus oregonensis, in response to a common predator, the red rock crab, Cancer productus. We conducted this study for 60 days, conducting day and night behavioral trials (with and without predators) four times a week. We found that crabs exposed to any amount of fluoxetine (3 or 30 ng/L) had increased activity levels relative to controls; however behaviors of 3 ng/L-spiked crabs were not always significantly different from controls. Among control crabs, day and night trials yielded similar results, where a clear response to the addition of the predator was observed. Crabs dosed with fluoxetine exhibited more foraging and active behaviors in the presence of the predator. Additionally, crabs spiked with fluoxetine at 30 ng/L had the greatest risk of mortality either by predation by red rock crabs or due to more aggressive behaviors among conspecifics. The results of this study shed light on a particularly unexplored area of contaminants research: how do psychoactive pharmaceuticals affect animal behavior when exposed to the low concentrations persisting in the aquatic environment for a prolonged period of time? Title: Pharmaceutical Contaminants as Stressors on Rocky Intertidal and Estuarine Organisms: a Case Study of Fluoxetine Author: Peters, Joseph Richard Year: 2016 Contaminants such as pharmaceuticals are of increasing concern due to their ubiquitous use and persistence in surface waters worldwide. Limited attention has been paid to the effects of pharmaceuticals on marine life, despite widespread detection of these contaminants in the marine environment. Of the existing studies, the majority assess the negative effects of pharmaceuticals over an exposure period of 30 days or less and focus on cellular and subcellular biomarkers. Longer studies are required to determine if chronic contaminant exposure poses risks to marine life at environmentally relevant concentrations. Also scarce in the literature is examination of whole organism effects to identify potential community-level consequences. Two long-term studies with the antidepressant pharmaceutical, fluoxetine (the active constituent in Prozac®) were conducted to determine whether nominal concentrations detected in estuarine and coastal environments affect organism health and interactions. First, we measured whole organism metrics in the California mussel, Mytilus californianus over a period of 107 days. Specifically, we measured algal clearance rates, growth, and condition indices for both reproductive and overall health. We found that fluoxetine negatively affects all measured characteristics, however many effects are mediated by length of exposure. Perhaps the most notable result was that mussels spiked with fluoxetine cleared less algae after 30 days of exposure. Reduced growth and condition indices likely are a consequence of improper nutrition among fluoxetine-treated mussels. Any level of fluoxetine significantly affected the gonadosomatic index after 47 days. The results from this study on mussels fill an important data gap, highlighting organism-level effects of chronic exposure periods; such data more explicitly identify the impacts of pharmaceuticals and other contaminants on marine communities and ecosystems. Fluoxetine has also been documented to affect the behavior of fish and invertebrates, including freshwater and marine bivalves, crustaceans, and fish. Given that other crustaceans exhibited increased activity levels under fluoxetine exposure, we hypothesized that this would subject them to greater predation risk. In our second exposure study, we assessed whether a similar range of fluoxetine concentrations used in the mussel study altered the risk behavior of the Oregon mud crab, Hemigrapsus oregonensis, in response to a common predator, the red rock crab, Cancer productus. We conducted this study for 60 days, conducting day and night behavioral trials (with and without predators) four times a week. We found that crabs exposed to any amount of fluoxetine (3 or 30 ng/L) had increased activity levels relative to controls; however behaviors of 3 ng/L-spiked crabs were not always significantly different from controls. Among control crabs, day and night trials yielded similar results, where a clear response to the addition of the predator was observed. Crabs dosed with fluoxetine exhibited more foraging and active behaviors in the presence of the predator. Additionally, crabs spiked with fluoxetine at 30 ng/L had the greatest risk of mortality either by predation by red rock crabs or due to more aggressive behaviors among conspecifics. The results of this study shed light on a particularly unexplored area of contaminants research: how do psychoactive pharmaceuticals affect animal behavior when exposed to the low concentrations persisting in the aquatic environment for a prolonged period of time? Close

• 9. [Article] Investigating the Link Between Surface Water and Groundwater in the Tule Lake Subbasin, Oregon and California

  Water allocation in the upper Klamath Basin of Oregon and California has been challenging. Irrigators have increasingly turned to groundwater to make up for surface water shortages because of shifts in ...

  Citation × Citation Citation Abstract Copy Title: Investigating the Link Between Surface Water and Groundwater in the Tule Lake Subbasin, Oregon and California Author: Pischel, Esther Maria Year: 2014 Water allocation in the upper Klamath Basin of Oregon and California has been challenging. Irrigators have increasingly turned to groundwater to make up for surface water shortages because of shifts in allocation toward in-stream flows for Endangered Species Act listed fishes. The largest increase in groundwater pumping has been in and around the Bureau of Reclamation's Klamath Irrigation Project, which includes the Tule Lake subbasin in the southern part of the upper Klamath Basin. Previous groundwater flow model simulations indicate that water level declines from pumping may result in decreased flow to agricultural drains in the Tule Lake subbasin. Agricultural drains on the Klamath Project are an important source of water for downstream irrigators and for the Tule Lake and Lower Klamath Lake National Wildlife Refuges. To better assess the impact of increased pumping on drain flow and on the water balance of the groundwater system, flow data from agricultural drains were evaluated to investigate the changes that have taken place in groundwater discharge to drains since pumping volumes increased. Additionally, a fine-grid groundwater model of the Tule Lake subbasin was developed based on the existing regional flow model. The fine-grid model has sufficient vertical and horizontal resolution to simulate vertical head gradients, takes advantage of time-series data from 38 observation wells for model calibration, and allows agricultural drains to be more explicitly represented. Results of the drain flow analysis show that the groundwater discharge to agricultural drains has decreased by approximately 4000 hectare-meters from the 1997-2000 average discharge. Most of this decrease takes place in the northern and southeastern portions of the subbasin. Results of the groundwater model show that the initial source of water to wells is groundwater storage. By 2006, approximately 56% of the water from wells is sourced from agricultural drains. Title: Investigating the Link Between Surface Water and Groundwater in the Tule Lake Subbasin, Oregon and California Author: Pischel, Esther Maria Year: 2014 Water allocation in the upper Klamath Basin of Oregon and California has been challenging. Irrigators have increasingly turned to groundwater to make up for surface water shortages because of shifts in allocation toward in-stream flows for Endangered Species Act listed fishes. The largest increase in groundwater pumping has been in and around the Bureau of Reclamation's Klamath Irrigation Project, which includes the Tule Lake subbasin in the southern part of the upper Klamath Basin. Previous groundwater flow model simulations indicate that water level declines from pumping may result in decreased flow to agricultural drains in the Tule Lake subbasin. Agricultural drains on the Klamath Project are an important source of water for downstream irrigators and for the Tule Lake and Lower Klamath Lake National Wildlife Refuges. To better assess the impact of increased pumping on drain flow and on the water balance of the groundwater system, flow data from agricultural drains were evaluated to investigate the changes that have taken place in groundwater discharge to drains since pumping volumes increased. Additionally, a fine-grid groundwater model of the Tule Lake subbasin was developed based on the existing regional flow model. The fine-grid model has sufficient vertical and horizontal resolution to simulate vertical head gradients, takes advantage of time-series data from 38 observation wells for model calibration, and allows agricultural drains to be more explicitly represented. Results of the drain flow analysis show that the groundwater discharge to agricultural drains has decreased by approximately 4000 hectare-meters from the 1997-2000 average discharge. Most of this decrease takes place in the northern and southeastern portions of the subbasin. Results of the groundwater model show that the initial source of water to wells is groundwater storage. By 2006, approximately 56% of the water from wells is sourced from agricultural drains. Close

• 10. [Article] Recreational Boats as Potential Vectors of Marine Organisms at an Invasion Hotspot

  With more than 200 aquatic nonindigenous species (NIS), San Francisco Bay (California, USA) is among the world’s most invaded harbors. Hard-substratum benthic (biofouling) organisms, which dominate NIS ...

  Citation × Citation Citation Abstract Copy Title: Recreational Boats as Potential Vectors of Marine Organisms at an Invasion Hotspot Author: Davidson, Ian C., Zabin, Chela J., Chang, Andrew L., Brown, Christopher W., Sytsma, Mark, Ruiz, Gregory M. Year: 2010 With more than 200 aquatic nonindigenous species (NIS), San Francisco Bay (California, USA) is among the world’s most invaded harbors. Hard-substratum benthic (biofouling) organisms, which dominate NIS richness, have arrived primarily as a result of shipping and aquaculture activity over past centuries. To date there has been no assessment of the leisure craft vector in the Bay. We aimed to characterize (1) biofouling on boats’ submerged surfaces and (2) boater behavior likely to affect the risk of NIS transfers. We used an underwater pole-cam, specimen collections, and a boater questionnaire to quantify the extent and composition of biofouling on recreational boats and to evaluate boater behavior at a subset of the Bay’s marinas. Several NIS, already established within the Bay, were recorded from vessel hulls, including the bryozoans Bugula neritina, Membranipora chesapeakensis and Watersipora sp., the ascidians Botrylloides violaceus, Styela clava and Ciona intestinalis, the polychaete Ficopomatus enigmaticus, and the sponge Clathria prolifera. Only 16% of questionnaire respondents had traveled to sites outside the Bay in the previous 12 mo. Frequency of hull painting and cleaning varied substantially, but we did not find strong patterns of biofouling extent associated with hull husbandry or boat usage. The potential for within-Bay and coastwise regional spread of NIS is high, and recreational boats probably interact in close proximity to other vectors (e.g. commercial ships), causing a ratchet effect of vector events; however, there remains a gap in understanding the levels and condition of biofouling on transient boats. Transient vessels from San Francisco Bay and other West Coast sites should be the focus of future studies to evaluate the extent to which organisms are being transferred among bays and how vector management could be applied to prevent NIS transfers and impacts. Title: Recreational Boats as Potential Vectors of Marine Organisms at an Invasion Hotspot Author: Davidson, Ian C., Zabin, Chela J., Chang, Andrew L., Brown, Christopher W., Sytsma, Mark, Ruiz, Gregory M. Year: 2010 With more than 200 aquatic nonindigenous species (NIS), San Francisco Bay (California, USA) is among the world’s most invaded harbors. Hard-substratum benthic (biofouling) organisms, which dominate NIS richness, have arrived primarily as a result of shipping and aquaculture activity over past centuries. To date there has been no assessment of the leisure craft vector in the Bay. We aimed to characterize (1) biofouling on boats’ submerged surfaces and (2) boater behavior likely to affect the risk of NIS transfers. We used an underwater pole-cam, specimen collections, and a boater questionnaire to quantify the extent and composition of biofouling on recreational boats and to evaluate boater behavior at a subset of the Bay’s marinas. Several NIS, already established within the Bay, were recorded from vessel hulls, including the bryozoans Bugula neritina, Membranipora chesapeakensis and Watersipora sp., the ascidians Botrylloides violaceus, Styela clava and Ciona intestinalis, the polychaete Ficopomatus enigmaticus, and the sponge Clathria prolifera. Only 16% of questionnaire respondents had traveled to sites outside the Bay in the previous 12 mo. Frequency of hull painting and cleaning varied substantially, but we did not find strong patterns of biofouling extent associated with hull husbandry or boat usage. The potential for within-Bay and coastwise regional spread of NIS is high, and recreational boats probably interact in close proximity to other vectors (e.g. commercial ships), causing a ratchet effect of vector events; however, there remains a gap in understanding the levels and condition of biofouling on transient boats. Transient vessels from San Francisco Bay and other West Coast sites should be the focus of future studies to evaluate the extent to which organisms are being transferred among bays and how vector management could be applied to prevent NIS transfers and impacts. Close