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• 1. [Article] The effect of cold acclimation on the temperature preference of the goldfish, Carassius auratus, and the brown bullhead, Ictalurus nebulosus

  Two species of fish, Carassius auratus and Ictalurus nebulosus, were subjected to cold acclimation regimes. Acclimation temperatures were slowly lowered to 3°c, then held for a period of time. At various ...

  Citation × Citation Citation Abstract Copy Title: The effect of cold acclimation on the temperature preference of the goldfish, Carassius auratus, and the brown bullhead, Ictalurus nebulosus Author: Lord, Alfred Year: 1987 Two species of fish, Carassius auratus and Ictalurus nebulosus, were subjected to cold acclimation regimes. Acclimation temperatures were slowly lowered to 3°c, then held for a period of time. At various times during this regime, fish were taken out and allowed to spend time in a temperature gradient to determine their preferred temperatures. Carassius were left in the gradient just long enough to determine a measure of the acute temperature preference, while Ictalurus were left in the temperature gradient for longer periods of time to observe any changes that might occur as the fish adjusted to selected temperatures. In both species of fish, lower acclimation temperatures and increased time spent at low temperatures caused a preference for lower temperatures in the gradient. Title: The effect of cold acclimation on the temperature preference of the goldfish, Carassius auratus, and the brown bullhead, Ictalurus nebulosus Author: Lord, Alfred Year: 1987 Two species of fish, Carassius auratus and Ictalurus nebulosus, were subjected to cold acclimation regimes. Acclimation temperatures were slowly lowered to 3°c, then held for a period of time. At various times during this regime, fish were taken out and allowed to spend time in a temperature gradient to determine their preferred temperatures. Carassius were left in the gradient just long enough to determine a measure of the acute temperature preference, while Ictalurus were left in the temperature gradient for longer periods of time to observe any changes that might occur as the fish adjusted to selected temperatures. In both species of fish, lower acclimation temperatures and increased time spent at low temperatures caused a preference for lower temperatures in the gradient. Close

• 2. [Article] Thermal Ecology and Movement in the Leopard Lizard, Gambelia Wislizeni, Baird and Girard

  Thermal ecology and movement of the Leopard Lizard, G. wistizeniwere studied at Frog Spring, adjacent to the Alvord Desert, and at various locations throughout the Alvord Basin. Seasonal activity is known ...

  Citation × Citation Citation Abstract Copy Title: Thermal Ecology and Movement in the Leopard Lizard, Gambelia Wislizeni, Baird and Girard Author: Abts, Marvin Lynn Year: 1976 Thermal ecology and movement of the Leopard Lizard, G. wistizeniwere studied at Frog Spring, adjacent to the Alvord Desert, and at various locations throughout the Alvord Basin. Seasonal activity is known to occur from early May to mid-October, but abundance. gradually decreases after late July. During favorable late spring, and summer weather lizards exhibit a bimodal pattern of diel activity with some activity occurring at all hours of the day between 0600 and 1800 H. At other times activity was unimodal. Emergence was apparently largely temperature dependent and did not occur until sand temperature approximated 21°C. Retreat appeared to be temperature independent, and was initiated by exogenous or endogenous stimuli at sand temperatures often above body temperatures acceptable for normal activity. For the period of study, juveniles exhibited diel activity patterns similar to adults, and were found during all hours in which adults were encountered. Body temperatures of field active lizards, largely affected by the immediate weather conditions, , ranged from 18.6° to 43.4°C. Because of a variety of thermoregulatory response (changes in location, posture, and orientation, color change, burrowing, shade-seeking, and panting), even during unfavorable weather conditions, most body temperatures fell within a narrower range. Nonetheless, the ability of field active lizards to precisely thermoregulate was limited, and the levels of body temperature maintained usually encompassed a broader range than those maintained in a thermal gradient. The mean body temperature of field active lizards during favorable weather conditions, and that of active lizards in a thermal gradient, did not differ significantly, and seemed to indicate a narrow range of body temperature preference. However, prevailing weather conditions were frequently unfavorable, thus limiting the extent of time during which selection of preferred body temperature could occur. Consequently, though activity depended on the attainment of certain temperature levels, G. wislizeni was forced to adapt to a broader range of body temperatures when performing most routine tasks. The necessity of precision thermoregulation is unclear, but apparently physiological efficiency was not greatly diminished over the broad range of body temperatures fiefd active lizards were often accepting. Relatively speaking, movement in G. wislizeni was extensive. Adult males occupied an average area more than twice the size of adult females and juveniles. Plotted polygons, constructed from sighting points, tended to be elongate. The extent to which lizards occupied a definite home range was uncertain. Adult males , continuously expanding the area occupied, probably lacked a home range. Adult females, demonstrating . little area expansion, perhaps maintained a home range. Territoriality by means of intraspecific display or aggression appeared negligible in G. wislizeni.Adult males did not demonstrate such behavior toward one another . Based on a limited extent of area overlap, adult females may have exhibited territoriality toward members of their sex. Movement per hour revealed results similar to area occupied, with adult males traveling an average distance of nearly three times as great as adult females and juveniles. Greatest distances frequently coincided with peak diel activity. Individual moves were frequent in adult males and juveniles, but infrequent in ~ adult females. Adult female long moves were similar in distance to adult males, but exogenous stimuli perhaps linked with home range maintenance restricted the frequency of such jaunts. Movement in G. wislizeni appeared independent of environmental thermal conditions when body temperature was within the range acceptable for normal activity. Diel movement appeared to be random in the species. Seasonal movement in juveniles, based on dispersion data, appeared directional. Title: Thermal Ecology and Movement in the Leopard Lizard, Gambelia Wislizeni, Baird and Girard Author: Abts, Marvin Lynn Year: 1976 Thermal ecology and movement of the Leopard Lizard, G. wistizeniwere studied at Frog Spring, adjacent to the Alvord Desert, and at various locations throughout the Alvord Basin. Seasonal activity is known to occur from early May to mid-October, but abundance. gradually decreases after late July. During favorable late spring, and summer weather lizards exhibit a bimodal pattern of diel activity with some activity occurring at all hours of the day between 0600 and 1800 H. At other times activity was unimodal. Emergence was apparently largely temperature dependent and did not occur until sand temperature approximated 21°C. Retreat appeared to be temperature independent, and was initiated by exogenous or endogenous stimuli at sand temperatures often above body temperatures acceptable for normal activity. For the period of study, juveniles exhibited diel activity patterns similar to adults, and were found during all hours in which adults were encountered. Body temperatures of field active lizards, largely affected by the immediate weather conditions, , ranged from 18.6° to 43.4°C. Because of a variety of thermoregulatory response (changes in location, posture, and orientation, color change, burrowing, shade-seeking, and panting), even during unfavorable weather conditions, most body temperatures fell within a narrower range. Nonetheless, the ability of field active lizards to precisely thermoregulate was limited, and the levels of body temperature maintained usually encompassed a broader range than those maintained in a thermal gradient. The mean body temperature of field active lizards during favorable weather conditions, and that of active lizards in a thermal gradient, did not differ significantly, and seemed to indicate a narrow range of body temperature preference. However, prevailing weather conditions were frequently unfavorable, thus limiting the extent of time during which selection of preferred body temperature could occur. Consequently, though activity depended on the attainment of certain temperature levels, G. wislizeni was forced to adapt to a broader range of body temperatures when performing most routine tasks. The necessity of precision thermoregulation is unclear, but apparently physiological efficiency was not greatly diminished over the broad range of body temperatures fiefd active lizards were often accepting. Relatively speaking, movement in G. wislizeni was extensive. Adult males occupied an average area more than twice the size of adult females and juveniles. Plotted polygons, constructed from sighting points, tended to be elongate. The extent to which lizards occupied a definite home range was uncertain. Adult males , continuously expanding the area occupied, probably lacked a home range. Adult females, demonstrating . little area expansion, perhaps maintained a home range. Territoriality by means of intraspecific display or aggression appeared negligible in G. wislizeni.Adult males did not demonstrate such behavior toward one another . Based on a limited extent of area overlap, adult females may have exhibited territoriality toward members of their sex. Movement per hour revealed results similar to area occupied, with adult males traveling an average distance of nearly three times as great as adult females and juveniles. Greatest distances frequently coincided with peak diel activity. Individual moves were frequent in adult males and juveniles, but infrequent in ~ adult females. Adult female long moves were similar in distance to adult males, but exogenous stimuli perhaps linked with home range maintenance restricted the frequency of such jaunts. Movement in G. wislizeni appeared independent of environmental thermal conditions when body temperature was within the range acceptable for normal activity. Diel movement appeared to be random in the species. Seasonal movement in juveniles, based on dispersion data, appeared directional. Close

• 3. [Article] Daily Temperature Fluctuations Unpredictably Influence Developmental Rate and Morphology at a Critical Early Larval Stage in a Frog

  Background: Environmental temperature has profound consequences for early amphibian development and many field and laboratory studies have examined this. Most laboratory studies that have characterized ...

  Citation × Citation Citation Abstract Copy Title: Daily Temperature Fluctuations Unpredictably Influence Developmental Rate and Morphology at a Critical Early Larval Stage in a Frog Author: Arrighi, Juliana M., Lencer, Ezra S., Jukar, Advait, Park, Daesik, Phillips, Patrick C., Kaplan, Robert H. Year: 2013 Background: Environmental temperature has profound consequences for early amphibian development and many field and laboratory studies have examined this. Most laboratory studies that have characterized the influence of temperature on development in amphibians have failed to incorporate the realities of diel temperature fluctuations (DTF), which can be considerable for pond-breeding amphibians. Results: We evaluated the effects of different ecologically relevant ranges of DTF compared with effects of constant temperatures on development of embryos and larvae of the Korean fire-bellied toad (Bombina orientalis). We constructed thermal reaction norms for developmental stage, snout- vent length, and tail length by fitting a Gompertz-Gaussian function to measurements taken from embryos after 66 hours of development in 12 different constant temperature environments between 14°C and 36°C. We used these reaction norms as null models to test the hypothesis that developmental effects of DTF are more than the sum of average constant temperature effects over the distribution of temperatures experienced. We predicted from these models that growth and differentiation would be positively correlated with average temperature at low levels of DTF but not at higher levels of DTF. We tested our prediction in the laboratory by rearing B. orientalis embryos at three average temperatures (20°C, 24°C, and 28°C) and four levels of thermal variation (0°C, 6°C, 13°C, and 20°C). Several of the observed responses to DTF were significantly different from both predictions of the model and from responses in constant temperature treatments at the same average temperatures. At an average temperature of 24°C, only the highest level of DTF affected differentiation and growth rates, but at both cooler and warmer average temperatures, moderate DTF was enough to slow developmental and tail growth rates. Conclusions: These results demonstrate that both the magnitude of DTF range and thermal averages need to be considered simultaneously when parsing the effects of changing thermal environments on complex developmental responses, particularly when they have potential functional and adaptive significance. Title: Daily Temperature Fluctuations Unpredictably Influence Developmental Rate and Morphology at a Critical Early Larval Stage in a Frog Author: Arrighi, Juliana M., Lencer, Ezra S., Jukar, Advait, Park, Daesik, Phillips, Patrick C., Kaplan, Robert H. Year: 2013 Background: Environmental temperature has profound consequences for early amphibian development and many field and laboratory studies have examined this. Most laboratory studies that have characterized the influence of temperature on development in amphibians have failed to incorporate the realities of diel temperature fluctuations (DTF), which can be considerable for pond-breeding amphibians. Results: We evaluated the effects of different ecologically relevant ranges of DTF compared with effects of constant temperatures on development of embryos and larvae of the Korean fire-bellied toad (Bombina orientalis). We constructed thermal reaction norms for developmental stage, snout- vent length, and tail length by fitting a Gompertz-Gaussian function to measurements taken from embryos after 66 hours of development in 12 different constant temperature environments between 14°C and 36°C. We used these reaction norms as null models to test the hypothesis that developmental effects of DTF are more than the sum of average constant temperature effects over the distribution of temperatures experienced. We predicted from these models that growth and differentiation would be positively correlated with average temperature at low levels of DTF but not at higher levels of DTF. We tested our prediction in the laboratory by rearing B. orientalis embryos at three average temperatures (20°C, 24°C, and 28°C) and four levels of thermal variation (0°C, 6°C, 13°C, and 20°C). Several of the observed responses to DTF were significantly different from both predictions of the model and from responses in constant temperature treatments at the same average temperatures. At an average temperature of 24°C, only the highest level of DTF affected differentiation and growth rates, but at both cooler and warmer average temperatures, moderate DTF was enough to slow developmental and tail growth rates. Conclusions: These results demonstrate that both the magnitude of DTF range and thermal averages need to be considered simultaneously when parsing the effects of changing thermal environments on complex developmental responses, particularly when they have potential functional and adaptive significance. Close

• 4. [Article] Temperature Dependent Transcription Initiation in Archaea: Interplay between Transcription Factor B and Promoter Sequence

  In Pyrococcus furiosus (Pfu), a hyperthermophile archaeon, two transcription factor Bs, TFB1 and TFB2 are encoded in the genomic DNA. TFB1 is the primary TFB in Pfu, and is homologous to transcription ...

  Citation × Citation Citation Abstract Copy Title: Temperature Dependent Transcription Initiation in Archaea: Interplay between Transcription Factor B and Promoter Sequence Author: Wu, Ming-Hsiao Year: 2014 In Pyrococcus furiosus (Pfu), a hyperthermophile archaeon, two transcription factor Bs, TFB1 and TFB2 are encoded in the genomic DNA. TFB1 is the primary TFB in Pfu, and is homologous to transcription factor IIB (TFIIB) in eukaryotes. TFB2 is proposed to be a secondary TFB that is compared to TFB1, TFB2 lacks the conserved B-finger / B-reader / B-linker regions which assist RNA polymerase in transcription start site selection and promoter opening functions respectively. P. furiosus, like all Archaea, encodes a single transcription factor E (TFE), that is homologous to the N-terminus of transcription factor II E (TFIIE) α subunit in eukaryotes. TFE stabilizes the transcription bubble when present, although it is not required for in vitro transcription. In this study, in vitro transcription is used to reveal how TFB2 responds to different temperature (65 °C, 70 °C, 75 °C, 80 °C, and 85 °C) at promoters for three different kinds of gene: non-temperature responsive, heat-shock induced, and cold-shock induced in the absence or presence of TFE. The activity of transcription complexes formed by TFB2 is always lower than by TFB1 in all temperatures and promoters. However, with heat-shock gene promoters, the activity of transcription complexes formed by TFB2 increases more than those formed with TFB1 with increasing temperatures. The temperature-dependent activities of TFB1 and TFB2 are similar with the non-temperature responsive gene promoter. With the cold-shock gene promoter, the activity of transcription complexes formed by both TFB1 and TFB2 has the highest activity in lower temperatures. When TFE is present, the activity of transcription complexes formed by TFB2 is enhanced with heat-shock gene promoters particularly at lower temperatures, and makes TFB2 behave more similarly to TFB1. With the non-temperature responsive gene promoter, TFB2 still behaves similarly to TFB1 when TFE is present. However, with the cold-shock gene promoter, most of the activity of transcription complexes formed by TFB1 and TFB2 remain the same, but only the activity of TFB1 decreases at 75 °C. The results suggest that TFB2 may play a role in heat-shock response through its increased sensitivity to temperature, and that TFE can modulate this temperature response. Title: Temperature Dependent Transcription Initiation in Archaea: Interplay between Transcription Factor B and Promoter Sequence Author: Wu, Ming-Hsiao Year: 2014 In Pyrococcus furiosus (Pfu), a hyperthermophile archaeon, two transcription factor Bs, TFB1 and TFB2 are encoded in the genomic DNA. TFB1 is the primary TFB in Pfu, and is homologous to transcription factor IIB (TFIIB) in eukaryotes. TFB2 is proposed to be a secondary TFB that is compared to TFB1, TFB2 lacks the conserved B-finger / B-reader / B-linker regions which assist RNA polymerase in transcription start site selection and promoter opening functions respectively. P. furiosus, like all Archaea, encodes a single transcription factor E (TFE), that is homologous to the N-terminus of transcription factor II E (TFIIE) α subunit in eukaryotes. TFE stabilizes the transcription bubble when present, although it is not required for in vitro transcription. In this study, in vitro transcription is used to reveal how TFB2 responds to different temperature (65 °C, 70 °C, 75 °C, 80 °C, and 85 °C) at promoters for three different kinds of gene: non-temperature responsive, heat-shock induced, and cold-shock induced in the absence or presence of TFE. The activity of transcription complexes formed by TFB2 is always lower than by TFB1 in all temperatures and promoters. However, with heat-shock gene promoters, the activity of transcription complexes formed by TFB2 increases more than those formed with TFB1 with increasing temperatures. The temperature-dependent activities of TFB1 and TFB2 are similar with the non-temperature responsive gene promoter. With the cold-shock gene promoter, the activity of transcription complexes formed by both TFB1 and TFB2 has the highest activity in lower temperatures. When TFE is present, the activity of transcription complexes formed by TFB2 is enhanced with heat-shock gene promoters particularly at lower temperatures, and makes TFB2 behave more similarly to TFB1. With the non-temperature responsive gene promoter, TFB2 still behaves similarly to TFB1 when TFE is present. However, with the cold-shock gene promoter, most of the activity of transcription complexes formed by TFB1 and TFB2 remain the same, but only the activity of TFB1 decreases at 75 °C. The results suggest that TFB2 may play a role in heat-shock response through its increased sensitivity to temperature, and that TFE can modulate this temperature response. Close

• 5. [Article] Effect of ethanol on thermoregulation in the goldfish, Carassius auratus

  In an attempt to elucidate the mechanism by which ethanol affects vertebrate thermoregulation, the effect of ethanol on temperature selection was studied in the goldfish, Carassius auratus. Ethanol was ...

  Citation × Citation Citation Abstract Copy Title: Effect of ethanol on thermoregulation in the goldfish, Carassius auratus Author: O'Connor, Candace Sharon Year: 1986 In an attempt to elucidate the mechanism by which ethanol affects vertebrate thermoregulation, the effect of ethanol on temperature selection was studied in the goldfish, Carassius auratus. Ethanol was administered to 10 to 15 g fish by mixing it in the water of a temperature gradient. The dose response curve was very steep between 0.5% (v/v) ethanol (no response) and 0.7% (significant lowering of selected temperature in treated fish). Fish were exposed to concentrations of ethanol as high as 1.7%, at which concentration most experimental fish lost their ability to swim upright in the water. At concentrations higher than 0.7%, the magnitude of the effect did not increase with increasing concentration of ethanol; treated animals continued to select temperatures about 2 C below temperatures selected by controls. Experiments alternating exposure to 1.0% ethanol and water showed that the rate of onset and disappearance of the ethanol effect was rapid (within 10 min). Other experiments exposing fish to 1.0% ethanol for up to 3 hr showed that the effect remained stable for this period of time. The thermoregulatory responses of fish are behavioral, and therefore relatively easy to observe and quantify. Ethanol produces a prompt, stable and reproducible depression of selected temperature in the goldfish. Because the temperature at which fish regulate is controlled by a central nervous system set point and not altered by effects on peripheral effector systems, it appears that ethanol may cause hypothermia in goldfish by directly acting to lower the set point. Title: Effect of ethanol on thermoregulation in the goldfish, Carassius auratus Author: O'Connor, Candace Sharon Year: 1986 In an attempt to elucidate the mechanism by which ethanol affects vertebrate thermoregulation, the effect of ethanol on temperature selection was studied in the goldfish, Carassius auratus. Ethanol was administered to 10 to 15 g fish by mixing it in the water of a temperature gradient. The dose response curve was very steep between 0.5% (v/v) ethanol (no response) and 0.7% (significant lowering of selected temperature in treated fish). Fish were exposed to concentrations of ethanol as high as 1.7%, at which concentration most experimental fish lost their ability to swim upright in the water. At concentrations higher than 0.7%, the magnitude of the effect did not increase with increasing concentration of ethanol; treated animals continued to select temperatures about 2 C below temperatures selected by controls. Experiments alternating exposure to 1.0% ethanol and water showed that the rate of onset and disappearance of the ethanol effect was rapid (within 10 min). Other experiments exposing fish to 1.0% ethanol for up to 3 hr showed that the effect remained stable for this period of time. The thermoregulatory responses of fish are behavioral, and therefore relatively easy to observe and quantify. Ethanol produces a prompt, stable and reproducible depression of selected temperature in the goldfish. Because the temperature at which fish regulate is controlled by a central nervous system set point and not altered by effects on peripheral effector systems, it appears that ethanol may cause hypothermia in goldfish by directly acting to lower the set point. Close

• 6. [Article] Febrile response and activity in the crayfish, Pacifasticus leniusculus trowbridgii

  Poikilothermic and endothermic animals demonstrate febrile response to infection with bacteria or to injection with endogenous pyrogen extract of Prostaglandin E1. Febrile response is measured in endotherms ...

  Citation × Citation Citation Abstract Copy Title: Febrile response and activity in the crayfish, Pacifasticus leniusculus trowbridgii Author: Fletcher, Kenneth A. Year: 1988 Poikilothermic and endothermic animals demonstrate febrile response to infection with bacteria or to injection with endogenous pyrogen extract of Prostaglandin E1. Febrile response is measured in endotherms as a relative change in metabolically achieved body temperature and in poikilotherms as an increase in selected temperatures relative to previously established preferred temperatures. Final preferendum change with environmental factors or associated physiological states. Title: Febrile response and activity in the crayfish, Pacifasticus leniusculus trowbridgii Author: Fletcher, Kenneth A. Year: 1988 Poikilothermic and endothermic animals demonstrate febrile response to infection with bacteria or to injection with endogenous pyrogen extract of Prostaglandin E1. Febrile response is measured in endotherms as a relative change in metabolically achieved body temperature and in poikilotherms as an increase in selected temperatures relative to previously established preferred temperatures. Final preferendum change with environmental factors or associated physiological states. Close

• 7. [Article] The metabolic cost of behavioral thermoregulation of body temperature in the northern alligator lizard Gerrhonotus coeruleus, and how it affects the classical concept of eurythermality

  The effect of total metabolic cost expenditures on the precision of behavioral thermoregulation was investigated for the purportedly eurythermic Northern Alligator lizard (Gerrhonotus coeruleus). An operant ...

  Citation × Citation Citation Abstract Copy Title: The metabolic cost of behavioral thermoregulation of body temperature in the northern alligator lizard Gerrhonotus coeruleus, and how it affects the classical concept of eurythermality Author: Campbell, James Dudley Year: 1981 The effect of total metabolic cost expenditures on the precision of behavioral thermoregulation was investigated for the purportedly eurythermic Northern Alligator lizard (Gerrhonotus coeruleus). An operant apparatus was designed to test metabolic output at different heat reinforcement magnitudes. The mean TB reflected in each trial was positively correlated to the length of reinforcement. The shuttle rate during each trial was inversely correlated to the length of reinforcement. The standard deviation and total metabolic costs did not vary significantly between trials undertaken at the same ambient temperature. Eurythermality in G. coeruleus is caused by fluctuations in preferred body temperature and not by fluctuations around this temperature. The metabolic cost of behavioral thermoregulation did not change with corresponding changes in reinforcement magnitude. This indicates that eurythermality is the recorded effect of lizards behaviorally regulating to different TB in a stenothermic manner, rather than of random TB fluctuations in a wide range of normal activity (the classical view of eurythermality). Title: The metabolic cost of behavioral thermoregulation of body temperature in the northern alligator lizard Gerrhonotus coeruleus, and how it affects the classical concept of eurythermality Author: Campbell, James Dudley Year: 1981 The effect of total metabolic cost expenditures on the precision of behavioral thermoregulation was investigated for the purportedly eurythermic Northern Alligator lizard (Gerrhonotus coeruleus). An operant apparatus was designed to test metabolic output at different heat reinforcement magnitudes. The mean TB reflected in each trial was positively correlated to the length of reinforcement. The shuttle rate during each trial was inversely correlated to the length of reinforcement. The standard deviation and total metabolic costs did not vary significantly between trials undertaken at the same ambient temperature. Eurythermality in G. coeruleus is caused by fluctuations in preferred body temperature and not by fluctuations around this temperature. The metabolic cost of behavioral thermoregulation did not change with corresponding changes in reinforcement magnitude. This indicates that eurythermality is the recorded effect of lizards behaviorally regulating to different TB in a stenothermic manner, rather than of random TB fluctuations in a wide range of normal activity (the classical view of eurythermality). Close

• 8. [Article] A Metabolic Hypothesis for the Evolution of Temperature Effects on the Arterial PCO2 and pH of Vertebrate Ectotherms

  Body temperature increases in ectothermic vertebrates characteristically lead to both increases in arterial PCO2 (PaCO2) and declines in resting arterial pH (pHa) of about 0.017 pH units/°C increase in ...

  Citation × Citation Citation Abstract Copy Title: A Metabolic Hypothesis for the Evolution of Temperature Effects on the Arterial PCO2 and pH of Vertebrate Ectotherms Author: Hillman, Stanley S., Hedrick, Michael Scott Year: 2017 Body temperature increases in ectothermic vertebrates characteristically lead to both increases in arterial PCO2 (PaCO2) and declines in resting arterial pH (pHa) of about 0.017 pH units/°C increase in temperature. This ‘alphastat’ pH pattern has previously been interpreted as being evolutionarily-driven by the maintenance of a constant protonation state on the imidazole moiety of histidine protein residues, hence stabilizing protein structure-function. Analysis of the existing data for interclass responses of ectothermic vertebrates show different degrees of PaCO2 increases and pH declines with temperature between the classes with reptiles>amphibians>fish. The PaCO2 at the temperature where maximal aerobic metabolism (VO2max) is achieved is significantly and positively correlated with temperature for all vertebrate classes. For ectotherms, the PaCO2 where VO2max is greatest is also correlated with VO2max indicating there is an increased driving force for CO2 efflux that is lowest in fish, intermediate in amphibians and highest in reptiles. The pattern of increased PaCO2 and the resultant reduction of pHa to increased body temperature would serve to increase CO2 efflux, O2 delivery, blood buffering capacity and maintain ventilatory scope. This represents a new hypothesis for the selective advantage of arterial pH regulation from a systems physiology perspective in addition to the advantages of maintenance of protein structure-function. Title: A Metabolic Hypothesis for the Evolution of Temperature Effects on the Arterial PCO2 and pH of Vertebrate Ectotherms Author: Hillman, Stanley S., Hedrick, Michael Scott Year: 2017 Body temperature increases in ectothermic vertebrates characteristically lead to both increases in arterial PCO2 (PaCO2) and declines in resting arterial pH (pHa) of about 0.017 pH units/°C increase in temperature. This ‘alphastat’ pH pattern has previously been interpreted as being evolutionarily-driven by the maintenance of a constant protonation state on the imidazole moiety of histidine protein residues, hence stabilizing protein structure-function. Analysis of the existing data for interclass responses of ectothermic vertebrates show different degrees of PaCO2 increases and pH declines with temperature between the classes with reptiles>amphibians>fish. The PaCO2 at the temperature where maximal aerobic metabolism (VO2max) is achieved is significantly and positively correlated with temperature for all vertebrate classes. For ectotherms, the PaCO2 where VO2max is greatest is also correlated with VO2max indicating there is an increased driving force for CO2 efflux that is lowest in fish, intermediate in amphibians and highest in reptiles. The pattern of increased PaCO2 and the resultant reduction of pHa to increased body temperature would serve to increase CO2 efflux, O2 delivery, blood buffering capacity and maintain ventilatory scope. This represents a new hypothesis for the selective advantage of arterial pH regulation from a systems physiology perspective in addition to the advantages of maintenance of protein structure-function. Close

• 9. [Article] The effect of body temperature on arteriovenous oxygen difference during rest and activity in the toad, Bufo marinus

  The relative contribution of arteriovenous oxygen (A-V O2) difference to thermally-induced and activity-induced changes in metabolic rate (VO2) were delineated in the toad, B. marinus. In conjunction, ...

  Citation × Citation Citation Abstract Copy Title: The effect of body temperature on arteriovenous oxygen difference during rest and activity in the toad, Bufo marinus Author: Palioca, Wayne Bryant Year: 1987 The relative contribution of arteriovenous oxygen (A-V O2) difference to thermally-induced and activity-induced changes in metabolic rate (VO2) were delineated in the toad, B. marinus. In conjunction, the influence of rest and activity, temperature, and removal of the pericardia! sac on separation efficiency (Es) in the anuran ventricle was determined by direct measurement of arterial and venous percent oxygen saturation (% saturation), using a microsampling technique. Title: The effect of body temperature on arteriovenous oxygen difference during rest and activity in the toad, Bufo marinus Author: Palioca, Wayne Bryant Year: 1987 The relative contribution of arteriovenous oxygen (A-V O2) difference to thermally-induced and activity-induced changes in metabolic rate (VO2) were delineated in the toad, B. marinus. In conjunction, the influence of rest and activity, temperature, and removal of the pericardia! sac on separation efficiency (Es) in the anuran ventricle was determined by direct measurement of arterial and venous percent oxygen saturation (% saturation), using a microsampling technique. Close

• 10. [Article] Behavioral Temperature Regulation in the Turtle Pseudemys Scripta Elegans

  A group of red-eared turtles (Pseudemys scripta elegans) was acclimated to 18-20˚C and artificial photo-period for 21 days. The animals were then individually placed in a thermal gradient and their selected ...

  Citation × Citation Citation Abstract Copy Title: Behavioral Temperature Regulation in the Turtle Pseudemys Scripta Elegans Author: Johnston, Michael H. Year: 1977 A group of red-eared turtles (Pseudemys scripta elegans) was acclimated to 18-20˚C and artificial photo-period for 21 days. The animals were then individually placed in a thermal gradient and their selected temperatures were continuously recorded for 24 hours. A second group of turtles was acclimatized to 7.5-11.0˚C and natural photoperiods for 28 days, and subsequently tested in the same manner as the first group. Lastly, the turtles in the first group, after being acclimated to 3.0˚C and the previous artificial photoperiod for 14 days, were retested in the gradient. Title: Behavioral Temperature Regulation in the Turtle Pseudemys Scripta Elegans Author: Johnston, Michael H. Year: 1977 A group of red-eared turtles (Pseudemys scripta elegans) was acclimated to 18-20˚C and artificial photo-period for 21 days. The animals were then individually placed in a thermal gradient and their selected temperatures were continuously recorded for 24 hours. A second group of turtles was acclimatized to 7.5-11.0˚C and natural photoperiods for 28 days, and subsequently tested in the same manner as the first group. Lastly, the turtles in the first group, after being acclimated to 3.0˚C and the previous artificial photoperiod for 14 days, were retested in the gradient. Close