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• 1. [Article] Multiplex SNP Discrimination

  Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, ...

  Citation × Citation Citation Abstract Copy Title: Multiplex SNP Discrimination Author: Fish, Daniel J., Horne, M. Todd, Searles, Robert P., Brewood, Greg P., Benight, Albert S. Year: 2007 Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection. Title: Multiplex SNP Discrimination Author: Fish, Daniel J., Horne, M. Todd, Searles, Robert P., Brewood, Greg P., Benight, Albert S. Year: 2007 Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection. Close