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• 1. [Article] Exposure of coho salmon (Oncorhynchus lisutch) to petroleum hydrocarbons : effect on lipid metabolism and aryl hydrocarbon hydroxylase activity

  Coho salmon (Oncorhynchus kisutch) were exposed to sublethal levels of a water-soluble fraction (WSF) of Cook Inlet crude oil for periods ranging from 18 to 31 days, at four acclimation temperatures. The ...

  Citation × Citation Citation Abstract Copy Title: Exposure of coho salmon (Oncorhynchus lisutch) to petroleum hydrocarbons : effect on lipid metabolism and aryl hydrocarbon hydroxylase activity Author: Caldarone, Elaine Marie Coho salmon (Oncorhynchus kisutch) were exposed to sublethal levels of a water-soluble fraction (WSF) of Cook Inlet crude oil for periods ranging from 18 to 31 days, at four acclimation temperatures. The fatty acid composition of phospholipids extracted from muscle, gill, liver, and brain tissues, and, at three of the temperatures, the activity of the hepatic microsomal aryl hydrocarbon hydroxylase (AHH) system in the salmon, were determined. Induction of the ARE system due to exposure of the fish to the toxicant was observed in the warm acclimated (20.0 C) fish only. With minor exceptions, the fatty acid composition of phospholipids in all of the tissues analyzed, including those from the warm acclimated fish, were unaltered by exposure of the fish to the crude oil WSF. In both control and exposed fish, AHH activity was greater in fish acclimated to 7.8 C than to 20.0 C. The phospholipids from both exposed and control cold acclimated fish contained a higher percentage of polyunsaturated fatty acids than the phospholipids from the corresponding warm acclimated fish. The studies reported here indicate that exposure of coho salmon (O. kisutch) to sublethal levels of petroleum hydrocarbons does not appreciably alter the fatty acid composition of tissue phospholipids or affect the normal change in fatty acid composition associated with a change in acclimation temperature. The data obtained are insufficient to permit any conclusion regarding the possibility of an interaction between the MFO system and the metabolsim of polyunsaturated fatty acids in oil exposed salmon. Title: Exposure of coho salmon (Oncorhynchus lisutch) to petroleum hydrocarbons : effect on lipid metabolism and aryl hydrocarbon hydroxylase activity Author: Caldarone, Elaine Marie Coho salmon (Oncorhynchus kisutch) were exposed to sublethal levels of a water-soluble fraction (WSF) of Cook Inlet crude oil for periods ranging from 18 to 31 days, at four acclimation temperatures. The fatty acid composition of phospholipids extracted from muscle, gill, liver, and brain tissues, and, at three of the temperatures, the activity of the hepatic microsomal aryl hydrocarbon hydroxylase (AHH) system in the salmon, were determined. Induction of the ARE system due to exposure of the fish to the toxicant was observed in the warm acclimated (20.0 C) fish only. With minor exceptions, the fatty acid composition of phospholipids in all of the tissues analyzed, including those from the warm acclimated fish, were unaltered by exposure of the fish to the crude oil WSF. In both control and exposed fish, AHH activity was greater in fish acclimated to 7.8 C than to 20.0 C. The phospholipids from both exposed and control cold acclimated fish contained a higher percentage of polyunsaturated fatty acids than the phospholipids from the corresponding warm acclimated fish. The studies reported here indicate that exposure of coho salmon (O. kisutch) to sublethal levels of petroleum hydrocarbons does not appreciably alter the fatty acid composition of tissue phospholipids or affect the normal change in fatty acid composition associated with a change in acclimation temperature. The data obtained are insufficient to permit any conclusion regarding the possibility of an interaction between the MFO system and the metabolsim of polyunsaturated fatty acids in oil exposed salmon. Close